Document Detail


Domain structure of the simian virus 40 core origin of replication.
MedLine Citation:
PMID:  3023900     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The simian virus 40 core origin of replication consists of nucleotides 5211 through 31. These 64 base pairs contain three functional domains with strict sequence requirements and two spacer regions with relaxed sequence specificity but precise positional constraints. The early domain extends for 10 contiguous base pairs between nucleotides 5211 and 5220. A 9-base pair spacer from sequences 5221 through 5229 separates the early domain from the 23-base pair central palindrome that directs the binding of T antigen. The late end of the core between nucleotides 12 and 31 also contains spacer and sequence-specific functions that are not yet completely mapped. We propose that the sequence-specific domains are interaction sites for viral and cellular proteins, determinants of DNA conformation, or both. The spacers would position these signals at required distances and rotations relative to one another.
Authors:
S Deb; A L DeLucia; C P Baur; A Koff; P Tegtmeyer
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  6     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  1986 May 
Date Detail:
Created Date:  1987-01-20     Completed Date:  1987-01-20     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1663-70     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Chromosome Deletion
DNA Replication*
DNA Restriction Enzymes
Genes, Viral*
Mutation
Plasmids
Simian virus 40 / genetics*
Virus Replication
Grant Support
ID/Acronym/Agency:
CA-09121/CA/NCI NIH HHS; CA-18808/CA/NCI NIH HHS; CA-28146/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
EC 3.1.21.-/DNA Restriction Enzymes
Comments/Corrections

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