Document Detail


Domain B of ARS307 contains two functional elements and contributes to chromosomal replication origin function.
MedLine Citation:
PMID:  7935479     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
ARS307 is highly active as a replication origin in its native location on chromosome III of Saccharomyces cerevisiae. Its ability to confer autonomous replication activity on plasmids requires the presence of an 11-bp autonomously replicating sequence (ARS) consensus sequence (ACS), which is also required for chromosomal origin function, as well as approximately 100 bp of sequence flanking the ACS called domain B. To further define the sequences required for ARS function, a linker substitution mutagenesis of domain B was carried out. The mutations defined two sequences, B1 and B2, that contribute to ARS activity. Therefore, like ARS1, domain B of ARS307 is composed of functional subdomains. Constructs carrying mutations in the B1 element were used to replace the chromosomal copy of ARS307. These mutations caused a reduction in chromosomal origin activity, demonstrating that the B1 element is required for efficient chromosomal origin function.
Authors:
J F Theis; C S Newlon
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  14     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  1994 Nov 
Date Detail:
Created Date:  1994-11-18     Completed Date:  1994-11-18     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7652-9     Citation Subset:  IM    
Affiliation:
Department of Microbiology and Molecular Genetics, UMD-New Jersey Medical School, Newark 07103.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Chromosomes, Fungal
Consensus Sequence
DNA Primers / genetics
DNA Replication / genetics*
DNA, Fungal / genetics
Molecular Sequence Data
Mutagenesis, Site-Directed
Replication Origin*
Saccharomyces cerevisiae / genetics*
Grant Support
ID/Acronym/Agency:
GM35679/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/DNA Primers; 0/DNA, Fungal
Comments/Corrections

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