| Domain B of ARS307 contains two functional elements and contributes to chromosomal replication origin function. | |
MedLine Citation:
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PMID: 7935479 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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ARS307 is highly active as a replication origin in its native location on chromosome III of Saccharomyces cerevisiae. Its ability to confer autonomous replication activity on plasmids requires the presence of an 11-bp autonomously replicating sequence (ARS) consensus sequence (ACS), which is also required for chromosomal origin function, as well as approximately 100 bp of sequence flanking the ACS called domain B. To further define the sequences required for ARS function, a linker substitution mutagenesis of domain B was carried out. The mutations defined two sequences, B1 and B2, that contribute to ARS activity. Therefore, like ARS1, domain B of ARS307 is composed of functional subdomains. Constructs carrying mutations in the B1 element were used to replace the chromosomal copy of ARS307. These mutations caused a reduction in chromosomal origin activity, demonstrating that the B1 element is required for efficient chromosomal origin function. |
Authors:
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J F Theis; C S Newlon |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Molecular and cellular biology Volume: 14 ISSN: 0270-7306 ISO Abbreviation: Mol. Cell. Biol. Publication Date: 1994 Nov |
Date Detail:
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Created Date: 1994-11-18 Completed Date: 1994-11-18 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 8109087 Medline TA: Mol Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 7652-9 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Molecular Genetics, UMD-New Jersey Medical School, Newark 07103. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Chromosomes, Fungal Consensus Sequence DNA Primers / genetics DNA Replication / genetics* DNA, Fungal / genetics Molecular Sequence Data Mutagenesis, Site-Directed Replication Origin* Saccharomyces cerevisiae / genetics* |
| Grant Support | |
ID/Acronym/Agency:
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GM35679/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/DNA, Fungal |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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