| Dolichol delays G1-arrest for one cell cycle in human fibroblasts subjected to depletion of serum or mevalonate. | |
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MedLine Citation:
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PMID: 1487489 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It is well-established that some product(s) or metabolite(s) of mevalonate is (are) critical for growth of mammalian cells. In the search for this (these) compound(s) it seems meaningful to distinguish between compounds needed for cell cycle progression in proliferating cells and compounds needed for growth activation of arrested cells. By using time-lapse video recording we have studied the possible regulatory role of cholesterol, dolichol and mevalonate in the cell cycle of human diploid fibroblasts (HDF). HDF, which are serum-dependent, were rapidly growth-arrested in the first part of G1 upon removal of serum factors. They also responded to mevinolin (an HMG CoA reductase inhibitor) by a similar G1-block, indicating that a mevalonate-derived product is involved in the G1-located cell cycle control of HDF. Interestingly, dolichol counteracted the G1-block caused by both types of treatment. Hence, the early G1-cells could traverse the remainder of the cell cycle and divide despite depletion of serum or mevalonate. We also demonstrated that addition of dolichol resulted in a significant decrease in the rate of protein degradation. This protein stabilizing effect may constitute the mechanism by which dolichol delays the G1-arrest of HDF. |
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Authors:
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O Larsson; J Wejde |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of cell science Volume: 103 ( Pt 4) ISSN: 0021-9533 ISO Abbreviation: J. Cell. Sci. Publication Date: 1992 Dec |
Date Detail:
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Created Date: 1993-02-24 Completed Date: 1993-02-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0052457 Medline TA: J Cell Sci Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1065-72 Citation Subset: IM |
Affiliation:
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Department of Tumor Pathology, Karolinska Hospital, Stockholm, Sweden. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Cycle
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drug effects Cells, Cultured Culture Media, Serum-Free / pharmacology* Dolichol / pharmacology* Fibroblasts / cytology, drug effects* Humans Hydroxymethylglutaryl-CoA Reductase Inhibitors Interphase / drug effects* Lovastatin / pharmacology Mevalonic Acid / pharmacology* Proteins / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Culture Media, Serum-Free; 0/Hydroxymethylglutaryl-CoA Reductase Inhibitors; 0/Proteins; 150-97-0/Mevalonic Acid; 2067-66-5/Dolichol; 75330-75-5/Lovastatin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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