Document Detail

Dogs cloned from fetal fibroblasts by nuclear transfer.
MedLine Citation:
PMID:  19135320     Owner:  NLM     Status:  MEDLINE    
Fetal fibroblasts have been considered as the prime candidate donor cells for the canine reproductive cloning by somatic cell nuclear transfer (SCNT) in regard to the future production of transgenic dogs, mainly due to their higher developmental competence and handling advantage in gene targeting. In this study, the cloning efficiency with canine fetal fibroblasts as donor cells was determined. A total of 50 presumptive cloned embryos were reconstructed, activated and transferred into the oviducts of naturally synchronous recipient bitches. While the fusion rate (76.9%) was similar to those of our earlier studies with adult fibroblasts as donor cells (73.9-77.1%), a high cloning efficiency (4.0%; 2 births/50 embryos transferred) was found compared to the previous success rate with adult fibroblasts (0.2-1.8%). The cloned beagles were healthy and genotypically identical to the donor fibroblast cells. This study shows that a fetal fibroblast cell would be an excellent donor for future production of transgenic dogs via gene targeting in this cell followed cloning using SCNT technology.
So Gun Hong; Goo Jang; Min Kyu Kim; Hyun Ju Oh; Jung Eun Park; Jung Taek Kang; Ok Jae Koo; Dae Yong Kim; Byeong Chun Lee
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-12-06
Journal Detail:
Title:  Animal reproduction science     Volume:  115     ISSN:  1873-2232     ISO Abbreviation:  Anim. Reprod. Sci.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-08-03     Completed Date:  2009-10-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7807205     Medline TA:  Anim Reprod Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  334-9     Citation Subset:  IM    
College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
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MeSH Terms
Animals, Genetically Modified
Cloning, Organism / methods,  veterinary*
Dogs / genetics*
Embryo Transfer / methods,  veterinary*
Fibroblasts / cytology,  physiology
Nuclear Transfer Techniques / veterinary*
Oocytes / cytology,  physiology*
Oviducts / physiology

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