| Does using a higher cutoff for the percentage of positive cells improve the specificity of HER-2 immunohistochemical analysis in breast carcinoma? | |
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MedLine Citation:
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PMID: 17951206 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study was performed to determine whether using a 30% cutoff for the proportion of HER-2+ cells would increase the specificity of HER-2 immunohistochemical analysis. Blinded to the HER-2 amplification status, 3 pathologists retrospectively reviewed HER-2-stained sections of breast carcinoma and considered cases to be positive for protein overexpression (3+) using 3 cutoff levels, 10%, 30%, and 50%, for the proportion of cells with intense uniform staining. Of 98 cases, 27 (28%) were positive by fluorescence in situ hybridization (FISH), and 32 (33%), 28 (29%), and 26 (27%) were considered positive for overexpression using cutoffs of 10%, 30%, and 50%, respectively. The specificity values of HER-2 immunohistochemical analysis were 82%, 86%, and 87% for the different cutoffs, and the concordance rates of 3+ cases with FISH were 59%, 64%, and 65%, for the cutoffs of 10%, 30%, and 50%, respectively. These findings seem to somehow support the recently proposed American Society of Clinical Oncology/College of American Pathologists guideline recommendations for HER-2 testing; nevertheless further studies are suggested. |
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Authors:
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Omar Hameed; David C Chhieng; Amy L Adams |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: American journal of clinical pathology Volume: 128 ISSN: 0002-9173 ISO Abbreviation: Am. J. Clin. Pathol. Publication Date: 2007 Nov |
Date Detail:
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Created Date: 2007-10-22 Completed Date: 2007-11-29 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0370470 Medline TA: Am J Clin Pathol Country: United States |
Other Details:
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Languages: eng Pagination: 825-9 Citation Subset: AIM; IM |
Affiliation:
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Department of Pathology, University of Alabama School of Medicine, Birmingham 35294-6823, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenocarcinoma
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chemistry*,
diagnosis Breast Neoplasms / chemistry*, diagnosis Cell Count Female Guidelines as Topic Humans Immunohistochemistry / methods In Situ Hybridization, Fluorescence Predictive Value of Tests Receptor, erbB-2 / analysis* Reproducibility of Results Retrospective Studies Tumor Markers, Biological / analysis* |
| Chemical | |
Reg. No./Substance:
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0/Tumor Markers, Biological; EC 2.7.10.1/ERBB2 protein, human; EC 2.7.10.1/Receptor, erbB-2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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