Document Detail


Does the tissue engineering architecture of poly(3-hydroxybutyrate) scaffold affects cell-material interactions?
MedLine Citation:
PMID:  22492575     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A critical element in tissue engineering involves the fabrication of a three-dimensional scaffold. The scaffold provides a space for new tissue formation, supports cellular ingrowth, and proliferation and mimics many roles of the extracellular matrix. Poly(3-hydroxybutyrate) (PHB) is the most thoroughly investigated member of the polyhydroxyalkanoates (PHAs) family that has various degrees of biocompatibility and biodegradability for tissue engineering applications. In this study, we fabricated PHB scaffolds by utilizing electrospinning and salt-leaching procedures. The behavior of monkey epithelial kidney cells (Vero) and mouse mesenchymal stem cells (mMSCs) on these scaffolds was compared by the MTS assay and scanning electron microscopy. Additionally, this study investigated the mechanical and physical properties of these scaffolds by measuring tensile strength and modulus, dynamic contact angle and porosity. According to our results, the salt-leached scaffolds showed more wettability and permeability, but inferior mechanical properties when compared with nanofibrous scaffolds. In terms of cell response, salt-leached scaffolds showed enhanced Vero cell proliferation, whereas both scaffolds responded similarly in the case of mMSCs proliferation. In brief, nanofibrous scaffolds can be a better substrate for cell attachment and morphology. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A 2012.
Authors:
Elahe Masaeli; Mohammad Morshed; Parsa Rasekhian; Saeed Karbasi; Khadije Karbalaie; Fereshte Karamali; Daryoush Abedi; Shahnaz Razavi; Abbas Jafarian-Dehkordi; Mohammad Hossein Nasr-Esfahani; Hossein Baharvand
Related Documents :
21566185 - Clonogenic neoblasts are pluripotent adult stem cells that underlie planarian regenerat...
21969095 - Imaging g-protein coupled receptor (gpcr)-mediated signaling events that control chemot...
21715255 - Cytotoxic effects of commercial wheatgrass and fiber towards human acute promyelocytic ...
21827875 - In search of representative models of human bone-forming cells for cytocompatibility st...
25212785 - Lead-free halide perovskite solar cells with high photocurrents realized through vacanc...
1483875 - Role of insulin-like growth factor-i in the autocrine regulation of cell growth in tt h...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-10
Journal Detail:
Title:  Journal of biomedical materials research. Part A     Volume:  -     ISSN:  1552-4965     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101234237     Medline TA:  J Biomed Mater Res A     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Affiliation:
Department of Textile Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran; Department of Cell and Molecular Biology, Royan Institute for Animal Biotechnology, Cell Science Research Center, ACECR, Isfahan 81589-68433, Iran.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Improved MR Imaging Detection of Cerebral Microbleeds More Accurately Identifies Persons with Vascul...
Next Document:  Catalytic Activation of Diazo Compounds Using Electron-Rich, Defined Iron Complexes for Carbene-Tran...