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Does the tissue engineering architecture of poly(3-hydroxybutyrate) scaffold affects cell-material interactions?
MedLine Citation:
PMID:  22492575     Owner:  NLM     Status:  Publisher    
A critical element in tissue engineering involves the fabrication of a three-dimensional scaffold. The scaffold provides a space for new tissue formation, supports cellular ingrowth, and proliferation and mimics many roles of the extracellular matrix. Poly(3-hydroxybutyrate) (PHB) is the most thoroughly investigated member of the polyhydroxyalkanoates (PHAs) family that has various degrees of biocompatibility and biodegradability for tissue engineering applications. In this study, we fabricated PHB scaffolds by utilizing electrospinning and salt-leaching procedures. The behavior of monkey epithelial kidney cells (Vero) and mouse mesenchymal stem cells (mMSCs) on these scaffolds was compared by the MTS assay and scanning electron microscopy. Additionally, this study investigated the mechanical and physical properties of these scaffolds by measuring tensile strength and modulus, dynamic contact angle and porosity. According to our results, the salt-leached scaffolds showed more wettability and permeability, but inferior mechanical properties when compared with nanofibrous scaffolds. In terms of cell response, salt-leached scaffolds showed enhanced Vero cell proliferation, whereas both scaffolds responded similarly in the case of mMSCs proliferation. In brief, nanofibrous scaffolds can be a better substrate for cell attachment and morphology. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A 2012.
Elahe Masaeli; Mohammad Morshed; Parsa Rasekhian; Saeed Karbasi; Khadije Karbalaie; Fereshte Karamali; Daryoush Abedi; Shahnaz Razavi; Abbas Jafarian-Dehkordi; Mohammad Hossein Nasr-Esfahani; Hossein Baharvand
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-10
Journal Detail:
Title:  Journal of biomedical materials research. Part A     Volume:  -     ISSN:  1552-4965     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101234237     Medline TA:  J Biomed Mater Res A     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Department of Textile Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran; Department of Cell and Molecular Biology, Royan Institute for Animal Biotechnology, Cell Science Research Center, ACECR, Isfahan 81589-68433, Iran.
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