Document Detail


Does periadventitial fat-derived nitric oxide play a role in improved saphenous vein graft patency in patients undergoing coronary artery bypass surgery?
MedLine Citation:
PMID:  17308436     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND/AIMS: The saphenous vein is commonly used for coronary artery bypass surgery but its patency is poor. Vascular damage occurs during conventional surgery. However, patency improves when the graft is harvested with minimal surgical trauma, partly due to preservation of vascular endothelial nitric oxide synthase (eNOS) and tissue sources of nitric oxide (NO), a factor possessing both dilatory and anti-proliferative properties. Apart from these grafts exhibiting an intact luminal endothelium they are harvested complete with a surrounding cushion of tissue, much of which is fat. METHODS: Immunostaining for eNOS was performed on vein graft sections and reverse-transcriptase polymerase chain reaction and Western blotting were used to identify eNOS mRNA and protein. NO synthase activity was measured using the citrulline assay. RESULTS: Immunohistochemistry identified eNOS staining of vein graft segments, including dense staining of the cushion of perivascular fat and associated structures surrounding the vein. eNOS protein was confirmed in both the vein and surrounding fat by Western blot analysis. Using the citrulline assay, the perivascular fat and underlying vein possessed comparable NO synthase activity. CONCLUSIONS: Our observations suggest that perivascular fat-derived NO plays a beneficial role in saphenous veins harvested atraumatically and used as grafts in patients undergoing coronary artery bypass surgery.
Authors:
Michael R Dashwood; Audrey Dooley; Xu Shi-Wen; David J Abraham; Domingos S R Souza
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Publication Detail:
Type:  Journal Article     Date:  2007-02-16
Journal Detail:
Title:  Journal of vascular research     Volume:  44     ISSN:  1018-1172     ISO Abbreviation:  J. Vasc. Res.     Publication Date:  2007  
Date Detail:
Created Date:  2007-04-13     Completed Date:  2007-05-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9206092     Medline TA:  J Vasc Res     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  175-81     Citation Subset:  IM    
Affiliation:
Department of Clinical Biochemistry, Royal Free and University College Medical School, London, UK. m.dashwood@medsch.ucl.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Adipose Tissue / metabolism*,  transplantation
Blotting, Western
Coronary Artery Bypass*
Humans
Immunohistochemistry
Nitric Oxide / metabolism*
Nitric Oxide Synthase Type III / genetics,  metabolism*
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Saphenous Vein / metabolism*,  physiopathology,  transplantation
Tissue and Organ Harvesting / methods*
Vascular Patency*
Chemical
Reg. No./Substance:
0/RNA, Messenger; 10102-43-9/Nitric Oxide; EC 1.14.13.39/NOS3 protein, human; EC 1.14.13.39/Nitric Oxide Synthase Type III

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