| Does level of ligation influence results in a murine biliary obstruction model? | |
| | |
MedLine Citation:
|
PMID: 7661299 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: Despite advances in perioperative management, patients with extrahepatic biliary obstruction still experience a high rate of complications and death after surgery. The rat is commonly used as an experimental animal for research in obstructive jaundice. Ligation of the rat bile duct high in the liver hilum is assumed to produce a more severe model of biliary obstruction than low ligation. The differences are attributed to the ability of the rat bile duct to dilate. Differences in level of ligation may, thus, explain some discrepancies between studies. MATERIALS AND METHODS: To test this hypothesis, female Lewis rats underwent high ligation (HL), low ligation (LL), and sham celiotomy. Colloidal carbon clearance, bilirubin, total serum bile acids, and hematocrit were measured 12 days later. Liver and spleen weight, presence or absence of ascites, infection, and adequacy of ligation were noted and the liver was processed for routine histology and electron microscopy. RESULTS: Although bilirubin levels were higher after HL than after LL, liver and spleen weight, total serum bile salts, and phagocytic constants K and alpha were not different between these two groups. Gross, histologic, and ultrastructural appearance did not differ between HL and LL groups. CONCLUSION: High ligation causes greater hyperbilirubinemia than low ligation, but does not alter other parameters including phagocytic constants. The present study does not confirm the hypothesis that HL creates a more severe model than LL; therefore, it is unlikely that differences in level of ligation explain variability in results between studies. |
| | |
Authors:
|
S Guidry; J B Grogan; C Subramony; J D Bass; V G Lockard; C E Scott-Conner |
Related Documents
:
|
16142379 - Repetitive and safe transgene expression in rat liver is achievable by adenoviral infus... 6403629 - Percutaneous penetration of three insecticides in rats: a comparison of two methods for... 12651609 - Hepatic artery and portal vein remodeling in rat liver: vascular response to selective ... 3967849 - Hydrochlorothiazide lowers urinary hydroxyproline in parathyroidectomized rats. 18612479 - Improvement of rat survival and liver mitochondrial function in biliary obstruction aft... 4072249 - Metabolism of the carcinogen 7-methylbenz[c]-acridine in the rat. 12213059 - 6-chloro-3-alkylamino-4h-thieno[3,2-e]-1,2,4-thiadiazine 1,1-dioxide derivatives potent... 2892159 - Further studies on the effect of glucose concentrations and other oxidizable substrates... 1768789 - A model for in vivo serial investigation of hepatic metabolism using 31p nuclear magnet... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: American journal of surgery Volume: 170 ISSN: 0002-9610 ISO Abbreviation: Am. J. Surg. Publication Date: 1995 Sep |
Date Detail:
|
Created Date: 1995-09-29 Completed Date: 1995-09-29 Revised Date: 2004-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 0370473 Medline TA: Am J Surg Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 289-91 Citation Subset: AIM; IM |
Affiliation:
|
Department of Surgery, University of Mississippi School of Medicine, Jackson, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Bile Acids and Salts / blood Bile Ducts / physiology, surgery* Bilirubin / blood Cholestasis / etiology* Disease Models, Animal Female Ligation / methods Liver / anatomy & histology Organ Size Phagocytosis Rats Rats, Inbred Lew Spleen / anatomy & histology |
| Chemical | |
Reg. No./Substance:
|
0/Bile Acids and Salts; 635-65-4/Bilirubin |
| Comments/Corrections | |
Comment In:
|
Am J Surg. 1995 Dec;170(6):701
[PMID:
7492030
]
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Pylorus-preserving gastrectomy with radical lymph node dissection based on anatomical variations of ...
Next Document: Nitric oxide: an overview.