Document Detail

Does high level production of SHV-type penicillinase confer resistance to ceftazidime in Enterobacteriaceae?
MedLine Citation:
PMID:  1624115     Owner:  NLM     Status:  MEDLINE    
We report the isolation of a clinical isolate of Klebsiella pneumoniae that showed resistance to ceftazidime (MIC: 8 micrograms/ml), susceptibility to aztreonam (MIC: 2 micrograms/ml) and cefotaxime (MIC: 0.015 micrograms/ml). A synergistic effect between clavulanic acid and ceftazidime or aztreonam against this strain was also observed. The strain hyperproduced SHV-1 penicillinase (990 U/g) which is encoded by a self-transferrable plasmid of at least 150 kb. That the ceftazidime-resistance phenotype could be due to hyperproduction of SHV-1 penicillinase is supported by the study of a spontaneous ceftazidime-resistant mutant in vitro obtained from an Escherichia coli strain containing plasmid p453 encoding the SHV-1. Indeed, this mutant hyperproducing SHV-1 (2200 U/g) was resistant to ceftazidime (MIC: 16 micrograms/ml) and aztreonam (MIC: 8 micrograms/ml) but susceptible to cefotaxime (MIC: 0.03 ng/ml). Clavulanic acid showed a synergistic effect when associated with ceftazidime or aztreonam. In contrast, the hyperproduction of TEM-1 (790 U/g) did not confer a ceftazidime- and aztreonam-resistant phenotype while hyperproduction of both TEM-1 and SHV-1 increased the resistance to amoxycillin/clavulanic acid and to cephalothin.
A Petit; H Ben Yaghlane-Bouslama; L Sofer; R Labia
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEMS microbiology letters     Volume:  71     ISSN:  0378-1097     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  1992 Apr 
Date Detail:
Created Date:  1992-08-10     Completed Date:  1992-08-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  89-94     Citation Subset:  IM    
Muséum National d'Histoire Naturelle, URA 401 CNRS, Paris, France.
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MeSH Terms
Ceftazidime / pharmacology*
Clavulanic Acid
Clavulanic Acids / pharmacology
Drug Interactions
Drug Resistance, Microbial*
Drug Therapy, Combination
Enterobacteriaceae / drug effects
Klebsiella pneumoniae / drug effects*
beta-Lactamases / metabolism*
Reg. No./Substance:
0/Clavulanic Acids; 58001-44-8/Clavulanic Acid; 78439-06-2/Ceftazidime; EC 3.5.2.-/beta-lactamase PIT-2; EC

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