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Does the combined intervention of soy isoflavones extract with mechanical vibration exert synergistic and trophic effects on bone tissue in rats?
MedLine Citation:
PMID:  23347380     Owner:  NLM     Status:  Publisher    
ABSTRACT Objective: to investigate the effects of soy isoflavones (Iso) and mechanical vibration (Vib) treatments alone or combined on bone extracellular matrix constituents of ovariectomized (Ovx) rats. Methods: 40 female Wistar rats at the age of 6 months were ovariectomized (Ovx) and 10 were sham-operated (sham) and after three months, the animals were divided in five groups: GI (sham); GII (Ovx); GIII - (Ovx-Iso) orally treated with Iso (200mg/kg) for 90 consecutive days; GIV - (Ovx-Vib) submitted to Vib for 90 days (5 days/week) and GV - (Ovx+Iso+Vib) treated with Iso plus Vib. After treatments, the rats were euthanized, and their femurs were removed for histological routine and biochemical study. Histological sections were stained with hematoxylin-eosin, picrosirius red and alcian blue. Shaft of femurs were submitted to biochemical assay and tibias were subjected to biophysical and biomechanical tests. Results: Treatments did not have significant effects in the trabecular bone volume, but the combined treatments showed trophic effects in the cortical bone width and area. Bone density and the content of organic material of the tibias were higher in the GIV and GV groups. The GV showed the highest presence of mature collagen fibers and the content of total GAGs, while the highest content of chondroitin sulfate and other sulfated GAGs were seen in the GIV group. Conclusion: the mechanical vibration treatment is more efficient than soy isoflavones in improving bone quality by the increase of bone density, the content sulfated GAGs and the presence of mature collagen fibers. In addition, the combined interventions have partial trophic and synergistic effects that are bone site specific in ovariectomized rats.
Rinaldo Florencio Silva; Miriam Aparecida Santos; Valquíria Pereira de Medeiros; Helena Bonciani Nader; Keico Okino Nonaka; Manuel Jesus Simões; Rejane Daniele Reginato
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-24
Journal Detail:
Title:  Climacteric : the journal of the International Menopause Society     Volume:  -     ISSN:  1473-0804     ISO Abbreviation:  Climacteric     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9810959     Medline TA:  Climacteric     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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