| Does carbonyl stress cause increased blood viscosity during storage? | |
| | |
MedLine Citation:
|
PMID: 20203369 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: The structural and biochemical changes to erythrocytes during storage, called 'storage lesion', are important factors that contribute to decreases in the efficacy and safety of blood transfusions. However, the biochemical mechanisms are only partly understood. STUDY DESIGN AND METHODS: Fresh whole blood in citrate phosphate dextrose anticoagulant was preserved in storage bags at 1-4 degrees C for over 20 days. Aliquots of stored blood were withdrawn for analysis at the 1st, 5th, 10th, 15th and 20th day of storage. Whole blood viscosity, plasma reactive carbonyl species, erythrocyte membrane protein carbonylation, protein (tryptophan) fluorescence and the contents of thiols were quantified concurrently. RESULTS: There were significant increases in blood viscosity and plasma reactive carbonyl species level during storage. There were significant time-dependent increases in membrane protein carbonylation, increases in protein (tryptophan) fluorescence and a decrease in the content of thiols. CONCLUSION: Reactive carbonyl species, by attacking the amino and/or sulfhydryl groups of erythrocyte membrane proteins, induce a series of structural alterations in erythrocytes. These lead to an increase in blood viscosity and reduce the efficacy and safety of storage and transfusion. |
| | |
Authors:
|
Guolin Li; Hong He; Han Yan; Qiong Zhao; Dazhong Yin |
Related Documents
:
|
3250629 - Erythrocyte aggregate rheology by transmitted and reflected light. 3784449 - Studies on haematocrit in peripheral arterial disease. 6540539 - Studies of the clinical pharmacology and therapeutic efficacy of pentoxifylline in peri... 17066169 - Piezoresistive cantilever based nanoflow and viscosity sensor for microchannels. 18405459 - Premature red blood cells have decreased aggregation and enhanced aggregability. 2003279 - Chronic blood hyperviscosity in subjects with acute stroke, transient ischemic attack, ... 14792339 - Citrate tolerance. 16797519 - Influence of hematocrit and localisation of punch in dried blood spots on levels of ami... 8037509 - Acute impairment of coronary blood flow by dynamic cardiomyoplasty. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Clinical hemorheology and microcirculation Volume: 44 ISSN: 1875-8622 ISO Abbreviation: Clin. Hemorheol. Microcirc. Publication Date: 2010 |
Date Detail:
|
Created Date: 2010-03-05 Completed Date: 2010-06-18 Revised Date: 2010-06-24 |
Medline Journal Info:
|
Nlm Unique ID: 9709206 Medline TA: Clin Hemorheol Microcirc Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 145-54 Citation Subset: IM |
Affiliation:
|
The Key Laboratory of Protein Chemistry and Developmental Biology of Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, Hunan, China. hnsdlgl@hunnu.edu.cn |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Blood
/
metabolism* Blood Preservation / methods*, standards Blood Transfusion Blood Viscosity* Citrates Glucose Humans Oxidative Stress / physiology* Protein Carbonylation / physiology* |
| Chemical | |
Reg. No./Substance:
|
0/Citrates; 50-99-7/Glucose; 51404-37-6/citrate phosphate dextrose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Alteration of serum thrombopoietin levels in patients with chronic hepatitis C under interferon ther...
Next Document: PI3K-AKT-mTOR pathway is dominant over androgen receptor signaling in prostate cancer cells.