Document Detail


Does blood pressure enhance solute transport in the bone lacunar-canalicular system?
MedLine Citation:
PMID:  20471508     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Solute transport through bone plays an important role in tissue metabolism and cellular mechanotransduction. Due to limited diffusion within the mineralized bone matrix, both mechanical loading and vascular pressure have been proposed to drive interstitial fluid flow within the lacunar-canalicular system (LCS); thereby augmenting solute diffusion in bone. Although blood supply is critical for bone nutrition, growth, and fracture healing, whether physiological blood pressures can drive significant fluid and solute convection remains controversial within the literature. The goal of this study was to directly test the hypothesis that in vivo blood pressures enhance solute transport in the bone LCS. Using a newly developed imaging approach based on fluorescence recovery after photobleaching (FRAP), we first measured the transport rate of sodium fluorescein (M.W. 376 Da) through the tibial LCS in four anesthetized mice (in the presence of vascular pressure). These data were then compared with the tracer transport rates at the same locations/lacunae after sacrifice (in the absence of vascular pressure). Using paired FRAP experiments we did not detect differences in tracer transport rates between bones from live anesthetized animals versus those in postmortem bodies (p>0.05, N=18). In a separate cohort of four anesthetized mice a mean jugular pulse pressure of approximately 10 mmHg at approximately 10 Hz was measured. Further theoretical analysis showed that for bones from both small and large animal species the blood pressure-driven convection of either small (376 Da) or large (43,000 Da) molecules was at least one order of magnitude smaller than diffusion under either normal or elevated pressure conditions. We conclude that despite the extreme importance of vasculature in bone physiology, vascular pressure itself does not enhance acute solute transport within the bone LCS. Therefore, mechanisms other than the vascular pressure-induced fluid flow such as altered biochemical factors may account for the bone adaptation associated with altered circulation. The present study helped clarify a long-standing controversy regarding vascular pressure-induced bone fluid flow and provided a better understanding of bone adaptation in both physiological and pathological conditions.
Authors:
Wen Li; Joseph D Gardinier; Christopher Price; Liyun Wang
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-05-13
Journal Detail:
Title:  Bone     Volume:  47     ISSN:  1873-2763     ISO Abbreviation:  Bone     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-12     Completed Date:  2010-10-05     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  8504048     Medline TA:  Bone     Country:  United States    
Other Details:
Languages:  eng     Pagination:  353-9     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Inc. All rights reserved.
Affiliation:
Graduate Program in Biomechanics and Movement Sciences, University of Delaware, Newark, DE 19716, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Transport
Blood Pressure / physiology*
Catheterization
Convection
Diffusion
Fluorescein / metabolism
Fluorescence Recovery After Photobleaching
Male
Mice
Mice, Inbred C57BL
Tibia / metabolism*
Grant Support
ID/Acronym/Agency:
AR054385/AR/NIAMS NIH HHS; P20 RR016458-076332/RR/NCRR NIH HHS; P20RR016458/RR/NCRR NIH HHS; R01 AR054385-03/AR/NIAMS NIH HHS; R01 AR054385-05/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
2321-07-5/Fluorescein
Comments/Corrections

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