Document Detail

Does additional hybridization also improve preimplantation genetic screening results?
MedLine Citation:
PMID:  21080815     Owner:  NLM     Status:  In-Process    
EVALUATION OF: Mir P, Rodrigo L, Mateu E et al. Improving FISH diagnosis for preimplantation genetic aneuploidy screening. Hum. Reprod. 25(7), 1812-1817 (2010). Mir et al. have evaluated the impact of additional hybridization rounds on FISH accuracy in preimplantation genetic screening (PGS) of aneuploidy. In a retrospective analysis of 1000 PGS cycles, embryo biopsy was performed on day 3. A greater number of embryos were diagnosed as chromosomally normal and were available for transfer after discarding 'false monosomies' and decreasing the number of noninformative embryos. The error rate for the FISH technique was 17.3% without and 5% with additional probes. A randomized controlled trial is needed to demonstrate improved clinical outcome. However, major obstacles in cleavage-stage PGS are chromosomal mosaicism and incomplete analysis. Therefore, polar body and/or trophectoderm analysis using 24 chromosome microarrays might be better alternatives.
Joep Pm Geraedts
Related Documents :
10734415 - A potential relationship between the 16;20 and 14;20 robertsonian translocations and lo...
20479065 - Snp microarray-based 24 chromosome aneuploidy screening demonstrates that cleavage-stag...
11673275 - Feasibility of producing porcine nuclear transfer embryos by using g2/m-stage fetal fib...
16727195 - Sex determination and milk protein genotyping of preimplantation stage bovine embryos u...
3838965 - Chromosomes of human sperm: variability among normal individuals.
3476255 - Patterns of chromosome breakage in nonhodgkin lymphoma: significance to gene alteration...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Expert review of molecular diagnostics     Volume:  10     ISSN:  1744-8352     ISO Abbreviation:  Expert Rev. Mol. Diagn.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-11-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101120777     Medline TA:  Expert Rev Mol Diagn     Country:  England    
Other Details:
Languages:  eng     Pagination:  981-5     Citation Subset:  IM    
Department of Genetics & Cell Biology, Research Institute GROW, Faculty of Health, Medicine & Life Sciences, Maastricht University, PO Box 5800, 6202 AZ Maastricht, The Netherlands.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Validation of a quality of life questionnaire in the Pacific Island.
Next Document:  Approaches to understanding adaptations of skin color variation by detecting gene-environment intera...