| Siderocalin/Lcn2/NGAL/24p3 does not drive apoptosis through gentisic acid mediated iron withdrawal in hematopoietic cell lines. | |
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MedLine Citation:
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PMID: 22928018 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Siderocalin (also lipocalin 2, NGAL or 24p3) binds iron as complexes with specific siderophores, which are low molecular weight, ferric ion-specific chelators. In innate immunity, siderocalin slows the growth of infecting bacteria by sequestering bacterial ferric siderophores. Siderocalin also binds simple catechols, which can serve as siderophores in the damaged urinary tract. Siderocalin has also been proposed to alter cellular iron trafficking, for instance, driving apoptosis through iron efflux via BOCT. An endogenous siderophore composed of gentisic acid (2,5-dihydroxybenzoic acid) substituents was proposed to mediate cellular efflux. However, binding studies reported herein contradict the proposal that gentisic acid forms high-affinity ternary complexes with siderocalin and iron, or that gentisic acid can serve as an endogenous siderophore at neutral pH. We also demonstrate that siderocalin does not induce cellular iron efflux or stimulate apoptosis, questioning the role siderocalin plays in modulating iron metabolism. |
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Authors:
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Colin Correnti; Vera Richardson; Allyson K Sia; Ashok D Bandaranayake; Mario Ruiz; Yohan Suryo Rahmanto; Žaklina Kovačević; Matthew C Clifton; Margaret A Holmes; Brett K Kaiser; Jonathan Barasch; Kenneth N Raymond; Des R Richardson; Roland K Strong |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2012-08-21 |
Journal Detail:
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Title: PloS one Volume: 7 ISSN: 1932-6203 ISO Abbreviation: PLoS ONE Publication Date: 2012 |
Date Detail:
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Created Date: 2012-08-28 Completed Date: 2013-01-29 Revised Date: 2013-04-15 |
Medline Journal Info:
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Nlm Unique ID: 101285081 Medline TA: PLoS One Country: United States |
Other Details:
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Languages: eng Pagination: e43696 Citation Subset: IM |
Affiliation:
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Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acute-Phase Proteins
/
chemistry,
pharmacology* Animals Apoptosis / drug effects* Cell Survival / drug effects Gentisates / metabolism* HeLa Cells Hematopoiesis* Humans Iron / metabolism* Lipocalins / chemistry, pharmacology* Mice Models, Molecular Protein Conformation Proto-Oncogene Proteins / chemistry, pharmacology* |
| Grant Support | |
ID/Acronym/Agency:
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AI117448/AI/NIAID NIH HHS; AI59432/AI/NIAID NIH HHS; DK55388/DK/NIDDK NIH HHS; DK58872/DK/NIDDK NIH HHS; HHSN272200700057C//PHS HHS; R01 AI011744/AI/NIAID NIH HHS; R01 DK073462/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Acute-Phase Proteins; 0/Gentisates; 0/LCN2 protein, human; 0/Lipocalins; 0/Proto-Oncogene Proteins; 7439-89-6/Iron; VP36V95O3T/2,5-dihydroxybenzoic acid |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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