Document Detail


Docosahexaenoic, arachidonic, palmitic, and oleic acids are differentially esterified into phospholipids of frog retina.
MedLine Citation:
PMID:  12324228     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Docosahexaenoic acid (22:6n-3) is highly enriched in the retina. To determine if retinal cells take up and metabolize fatty acids in a specific manner, retinas from Rana pipiens were incubated for 3 h with an equimolar mixture of tritiated 22:6n-3, arachidonic acid (20:4n-6), palmitic acid, and oleic acid. The radiolabeling of retinal lipids was determined and compared to the endogenous fatty acid content of the lipids. The results showed that in most, but not all, cases, the relative labeling with the four precursor fatty acids was similar to their relative abundance in each glycerolipid. Thus, during retinal glycerolipid synthesis, either through de novo or acyl exchange reactions, fatty acids are incorporated in proportions reflecting their steady-state mass levels. Since other studies with labeled glycerol have shown greater differences between early labeling patterns and molecular species mass, the final incorporation we report may be due primarily to acyl exchange reactions.
Authors:
R E Martin; S A Hopkins; R Steven Brush; C Williamson; H Chen; R E Anderson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Prostaglandins, leukotrienes, and essential fatty acids     Volume:  67     ISSN:  0952-3278     ISO Abbreviation:  Prostaglandins Leukot. Essent. Fatty Acids     Publication Date:    2002 Aug-Sep
Date Detail:
Created Date:  2002-09-26     Completed Date:  2003-09-05     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8802730     Medline TA:  Prostaglandins Leukot Essent Fatty Acids     Country:  Scotland    
Other Details:
Languages:  eng     Pagination:  105-11     Citation Subset:  IM    
Copyright Information:
Copyright 2002 Elsevier Science Ltd.
Affiliation:
Department of Cell Biology, Oklahoma City, OK, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Arachidonic Acid / metabolism*
Chromatography, Thin Layer
Docosahexaenoic Acids / metabolism*
Esterification
Oleic Acids / metabolism*
Palmitic Acid / metabolism*
Phospholipids / chemistry,  metabolism*
Rana pipiens / metabolism*
Retina / chemistry,  metabolism*
Grant Support
ID/Acronym/Agency:
EY00871/EY/NEI NIH HHS; EY04149/EY/NEI NIH HHS; EY12190/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/Oleic Acids; 0/Phospholipids; 25167-62-8/Docosahexaenoic Acids; 506-32-1/Arachidonic Acid; 57-10-3/Palmitic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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