Document Detail

Docosahexaenoic acid selectively augments muscarinic stimulation of epithelial Cl- secretion.
MedLine Citation:
PMID:  12788663     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: We investigated the effect of various fatty acids on electrogenic chloride secretion in T84 cells, a model for intestinal epithelium. MATERIALS AND METHODS: T84 intestinal epithelial cells grown on permeable supports were studied by conventional current-voltage clamping. Membrane lipids from T84 cells were extracted, transmethylated, and analyzed by gas chromatography. Lipid extracts were fractionated into nonpolar, free fatty acids, and phospholipids by amynopropil column chromatography. RESULTS: Docosahexaenoic acid (DHA) but not eicosapentanoic acid or other fatty acids selectively enhanced the secretory response to the muscarinic agonist carbachol but not the response to other Ca2+ agonists (histamine, thapsigargin, or ionomycin) or the response to the cAMP agonist forskolin. The ability of DHA to augment Cl- secretion appeared to correlate closer with free DHA levels than with membrane-bound DHA. Other effects of DHA on T84 cells included a reduction in transepithelial resistance (a measure of barrier function), actions that were dissociated from the effect on Cl- secretion. CONCLUSION: The results suggest that DHA, which has been shown to reverse organ pathology in experimental cystic fibrosis, may selectively affect agonist-regulated transport events and other fundamental properties of epithelial cells.
Isabel Calvo Del Castillo; Juan G Alvarez; Steven D Freedman; Mario Ollero; Leonardo Claros; J Cecilia Song; James Yoo; Jeffrey B Matthews
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of surgical research     Volume:  110     ISSN:  0022-4804     ISO Abbreviation:  J. Surg. Res.     Publication Date:  2003 Apr 
Date Detail:
Created Date:  2003-06-05     Completed Date:  2003-07-08     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0376340     Medline TA:  J Surg Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  338-43     Citation Subset:  IM    
Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
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MeSH Terms
Carbachol / metabolism*
Cell Line
Chlorides / metabolism*
Cystic Fibrosis / metabolism
Docosahexaenoic Acids / metabolism*
Intestinal Mucosa / metabolism,  secretion*
Models, Biological
Muscarinic Agonists / metabolism*
Grant Support
Reg. No./Substance:
0/Chlorides; 0/Muscarinic Agonists; 25167-62-8/Docosahexaenoic Acids; 51-83-2/Carbachol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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