Document Detail

Docosahexaenoic acid-containing phosphatidylethanolamine in the external layer of liposomes protects docosahexaenoic acid from 2,2'-azobis(2-aminopropane)dihydrochloride-mediated lipid peroxidation.
MedLine Citation:
PMID:  12559986     Owner:  NLM     Status:  MEDLINE    
We have proposed that incorporation of docosahexaenoic acid (DHA) into phosphatidylethanolamine (PE) might enhance resistance to lipid peroxidation in vivo. In this study, we examined the relationship between the transbilayer distribution of PE and the oxidative stability of DHA in PE. Liposomes composed of a phospholipid mixture were used as models for biological membranes. To modulate the transbilayer distribution of PE obtained from the liver of rats fed DHA (PE-DHA), we used phosphatidylcholine (PC) with two types of acyl chain region: dipalmitoyl (PC16:0) or dioleoyl (PC18:1). The proportion of PE-DHA in the liposomal external layer was significantly higher in liposomes containing PC18:1 than in those containing PC16:0. This tendency was more pronounced in liposomes extruded using a polycarbonate filter with smaller pore sizes. Additionally, PE-DHA in the external layer of liposomes prepared using a filter with smaller pore sizes could protect DHA itself from 2,2(')-azobis(2-aminopropane)dihydrochloride-mediated lipid peroxidation.
Kazuhiro Kubo; Seiji Sekine; Morio Saito
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  410     ISSN:  0003-9861     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  2003 Feb 
Date Detail:
Created Date:  2003-01-31     Completed Date:  2003-02-21     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  United States    
Other Details:
Languages:  eng     Pagination:  141-8     Citation Subset:  IM    
Division of Food Science, Department of Food Science Research for Health, Independent Administrative Institution, National Institute of Health and Nutrition, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8636, Japan.
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MeSH Terms
Amidines / pharmacology
Docosahexaenoic Acids / chemistry*,  metabolism,  pharmacology
Fatty Acids
Lipid Bilayers
Lipid Peroxidation / drug effects
Liposomes / chemistry*,  metabolism
Liver / drug effects,  metabolism
Oxidants / pharmacology
Phosphatidylcholines / chemistry,  metabolism
Phosphatidylethanolamines / chemistry*,  metabolism
Rats, Sprague-Dawley
Structure-Activity Relationship
Trinitrobenzenesulfonic Acid / chemistry
Reg. No./Substance:
0/Amidines; 0/Fatty Acids; 0/Lipid Bilayers; 0/Liposomes; 0/Oxidants; 0/Phosphatidylcholines; 0/Phosphatidylethanolamines; 13217-66-8/2,2'-azobis(2-amidinopropane); 2508-19-2/Trinitrobenzenesulfonic Acid; 25167-62-8/Docosahexaenoic Acids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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