Document Detail

Docosahexaenoic and eicosapentaenoic acids segregate differently between raft and nonraft domains.
MedLine Citation:
PMID:  22853900     Owner:  NLM     Status:  MEDLINE    
Omega-3 polyunsaturated fatty acids (n-3 PUFA), enriched in fish oils, are increasingly recognized to have potential benefits for treating many human afflictions. Despite the importance of PUFA, their molecular mechanism of action remains unclear. One emerging hypothesis is that phospholipids containing n-3 PUFA acyl chains modify the structure and composition of membrane rafts, thus affecting cell signaling. In this study the two major n-3 PUFA found in fish oils, eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids, are compared. Using solid-state (2)H NMR spectroscopy we explored the molecular organization of 1-[(2)H(31)]palmitoyl-2-eicosapentaenoylphosphatidylcholine (PEPC-d(31)) and 1-[(2)H(31)]palmitoyl-2-docosahexaenoylphosphatidylcholine (PDPC-d(31)) in mixtures with sphingomyelin (SM) and cholesterol (chol). Our results indicate that whereas both PEPC-d(31) and PDPC-d(31) can accumulate into SM-rich/chol-rich raftlike domains, the tendency for DHA to incorporate into rafts is more than twice as great as for EPA. We propose that DHA may be the more bioactive component of fish oil that serves to disrupt lipid raft domain organization. This mechanism represents an evolution in the view of how PUFA remodel membrane architecture.
Justin A Williams; Shawn E Batten; Mitchel Harris; Benjamin Drew Rockett; Saame Raza Shaikh; William Stillwell; Stephen R Wassall
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-07-17
Journal Detail:
Title:  Biophysical journal     Volume:  103     ISSN:  1542-0086     ISO Abbreviation:  Biophys. J.     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-08-02     Completed Date:  2012-12-10     Revised Date:  2013-07-21    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  228-37     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Biophysical Society. Published by Elsevier Inc. All rights reserved.
Department of Physics, Indiana University-Purdue University Indianapolis, Indianapolis, Indiana, USA.
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MeSH Terms
Cholesterol / chemistry
Docosahexaenoic Acids / chemistry*
Eicosapentaenoic Acid / chemistry*
Magnetic Resonance Spectroscopy
Membrane Microdomains / chemistry*
Membranes, Artificial
Oleic Acids / chemistry
Phosphatidylcholines / chemistry
Sphingomyelins / chemistry
Grant Support
Reg. No./Substance:
0/1-palmitoyl-2-elaidoylphosphatidylcholine; 0/Detergents; 0/Membranes, Artificial; 0/Oleic Acids; 0/Phosphatidylcholines; 0/Sphingomyelins; 1553-41-9/Eicosapentaenoic Acid; 25167-62-8/Docosahexaenoic Acids; 57-88-5/Cholesterol; 59403-54-2/1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine; TE895536Y5/1-palmitoyl-2-oleoylphosphatidylcholine

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