| Docosahexaenoate and eicosapentaenoate reverse cystic fibrosis-related fatty acid abnormalities by suppressing fatty acid desaturase expression and activity. | |
| | |
MedLine Citation:
|
PMID: 22095831 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Patients and models of cystic fibrosis (CF) exhibit consistent abnormalities of polyunsaturated (PUFA) fatty acid composition, including decreased linoleate (LA) and docosahexaenoate (DHA) and variably increased arachidonate (AA), related in part to increased expression and activity of fatty acid desaturases. These abnormalities and the consequent CF-related pathologic manifestations can be reversed in CF mouse models by dietary supplementation with DHA. However, the mechanism is unknown. This study investigates this mechanism by measuring the effect of exogenous DHA and eicosapentaenoate (EPA) supplementation on fatty acid composition and metabolism, as well as on metabolic enzyme expression, in a cell culture model of CF. We found that both DHA and EPA suppress the expression and activity of Δ5- and Δ6-desaturases, leading to decreased flux through the n-3 and n-6 PUFA metabolic pathways and decreased production of AA. The findings also uncover other metabolic abnormalities, including increased fatty acid uptake and markedly increased retroconversion of DHA to EPA, in CF cells. These results indicate that the fatty acid abnormalities of CF are related to intrinsic alterations of PUFA metabolism and that they may be reversed by supplementation with DHA and EPA. |
| | |
Authors:
|
Sarah W Njoroge; Michael Laposata; Waddah Katrangi; Adam C Seegmiller |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-11-16 |
Journal Detail:
|
Title: Journal of lipid research Volume: - ISSN: 0022-2275 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
|
Created Date: 2011-11-18 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Vanderbilty University Medical Center, United States; |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: From Pluri- to Multipotency via Vascular Pericytes; Are Mural Cells Guardians of Stemness?
Next Document: Primary fatty acid amide metabolism:conversion of fatty acids and an Ethanolamine in N18TG2 and SCP ...