Document Detail


Do gonadotropin-releasing hormone, tyrosine hydroxylase-, and beta-endorphin-immunoreactive neurons contain estrogen receptors? A double-label immunocytochemical study in the Suffolk ewe.
MedLine Citation:
PMID:  8102098     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We used double label immunocytochemistry to examine the brains of ovariectomized ewes and determine whether GnRH, tyrosine hydroxylase-(TH), and beta-endorphin-immunoreactive (IR) neurons contain IR-estrogen receptors (ER). Because of their possible importance as a target for the feedback actions of estradiol, we also examined the presence of nuclear ER in LH-IR cells of the pars tuberalis of the pituitary. Although preoptic GnRH neurons were frequently in close proximity to ER-IR cells, only one out of approximately 1000 GnRH cells examined was found to coexpress ER. In contrast, in the arcuate nucleus and vicinity, 3-5% of TH cells and 15-20% of beta-endorphin cells contained ER. Virtually all LH-IR cells, seen predominantly in the ventral portion of the pars tuberalis, coexpressed ER. These results suggest that in sheep as in rodents, the influence of estradiol on the reproductive neuroendocrine system is not directly mediated by GnRH neurons, but instead is conveyed to GnRH cells via presynaptic afferents. Subsets of TH- and beta-endorphin-IR cells which coexpress ER are two candidates for relaying gonadal steroid signals to GnRH cells. At the level of the pituitary, the feedback actions of estradiol may be expressed directly upon the gonadotroph.
Authors:
M N Lehman; F J Karsch
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Endocrinology     Volume:  133     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1993 Aug 
Date Detail:
Created Date:  1993-09-03     Completed Date:  1993-09-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  887-95     Citation Subset:  AIM; IM    
Affiliation:
Department of Anatomy and Cell Biology, University of Cincinnati College of Medicine, Ohio 45267-0521.
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MeSH Terms
Descriptor/Qualifier:
Animals
Female
Fluorescent Antibody Technique
Gonadotropin-Releasing Hormone / analysis*
Immunoenzyme Techniques
Luteinizing Hormone / analysis
Neurons / chemistry*
Receptors, Estrogen / analysis*
Sheep
Tissue Distribution
Tyrosine 3-Monooxygenase / analysis*
beta-Endorphin / analysis*
Grant Support
ID/Acronym/Agency:
HD-18337/HD/NICHD NIH HHS; HD-21968/HD/NICHD NIH HHS; P30-HD-18258/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Receptors, Estrogen; 33515-09-2/Gonadotropin-Releasing Hormone; 60617-12-1/beta-Endorphin; 9002-67-9/Luteinizing Hormone; EC 1.14.16.2/Tyrosine 3-Monooxygenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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