Document Detail

Dna-end binding activity of Ku in synchronized cells.
MedLine Citation:
PMID:  10736189     Owner:  NLM     Status:  MEDLINE    
Three different types of cells were synchronized by various methods and DNA-end binding (DEB) activities of Ku were compared with asynchronous controls. In CHO K1 cells synchronized in G1 phase by serum starvation and in S phase by serum refeeding, DEB activity was reduced in S cells but remained unchanged in G1 cells. However, the same type of cells synchronized in G1/S phase by double thymidine block and in S phase by releasing the blockage, have the same DEB activity as asynchronous controls. A similar result was found in RKO and HeLa cells synchronized by the latter method. Arresting cells in mitosis with nocodazole also generated different cell cycle effects. Ku activity was reduced in CHO K1 and RKO cells, but not in HeLa cells after treatment with nocodazole. In phase-enriched cells separated by centrifugal elutriation, DEB activities were similar at different stages of the cell cycle in all three types of cells. Thus, different synchronization procedures can give very different values of Ku activity in a cell type-dependent manner. Results from elutriated cells are consistent, and suggest DEB activity of Ku does not change with the cell cycle.
L F Chou; W G Chou
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biology international     Volume:  23     ISSN:  1065-6995     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  1999  
Date Detail:
Created Date:  2000-04-27     Completed Date:  2000-04-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  663-70     Citation Subset:  IM    
Copyright Information:
Copyright 1999 Academic Press.
Department of Life Science, National Tsin-Hua University, Hsinchu, Taiwan, R.O.C.
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MeSH Terms
Antigens, Nuclear*
Antineoplastic Agents / pharmacology*
Blood Proteins / pharmacology
CHO Cells
Cell Separation
Colorectal Neoplasms
DNA / metabolism
DNA Helicases*
DNA-Binding Proteins / metabolism*
Hela Cells
Mitosis / drug effects*,  physiology
Nocodazole / pharmacology*
Nuclear Proteins / metabolism*
Thymidine / pharmacology
Reg. No./Substance:
0/Antigens, Nuclear; 0/Antineoplastic Agents; 0/Blood Proteins; 0/DNA-Binding Proteins; 0/Ku autoantigen; 0/Nuclear Proteins; 0/XRCC5 protein, human; 31430-18-9/Nocodazole; 50-89-5/Thymidine; 9007-49-2/DNA; EC 3.6.1.-/DNA Helicases

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