Document Detail

DmSAS is required for sialic acid biosynthesis in cultured Drosophila third instar larvae CNS neurons.
MedLine Citation:
PMID:  21919466     Owner:  NLM     Status:  MEDLINE    
Sialylation is an important carbohydrate modification of glycoconjugates that has been shown to modulate many cellular/molecular interactions in vertebrates. In Drosophila melanogaster (Dm), using sequence homology, several enzymes of the sialylation pathway have been cloned and their function tested in expression systems. Here we investigated whether sialic acid incorporation in cultured Dm central nervous system (CNS) neurons required endogenously expressed Dm sialic acid synthase (DmSAS). We compared neurons derived from wild type Dm larvae with those containing a DmSAS mutation (148 bp deletion). The ability of these cells to produce Sia5NAz (sialic acid form) from Ac(4)ManNAz (azide-derivatized N-acetylmannosamine) and incorporate it into their glycoconjugates was measured by tagging the azide group of Sia5NAz with fluorescent agents via Click-iT chemistry. We found that most of the wild type Dm CNS neurons incorporated Sia5NAz into their glycoconjugates. Sialic acid incorporation was higher at the soma than at the neurite and could also be detected at perinuclear regions and the plasma membrane. In contrast, neurons from the DmSAS mutant did not incorporate Sia5NAz unless DmSAS was reintroduced (rescue mutant). Most of the neurons expressed α2,6-sialyltransferase. These results confirm that the mutation was a null mutation and that no redundant sialic acid biosynthetic activity exists in Dm cells, i.e., there is only one DmSAS. They also provide the strongest proof to date that DmSAS is a key enzyme in the biosynthesis of sialic acids in Dm CNS neurons, and the observed subcellular distribution of the newly synthesized sialic acids offers insights into their biological function.
Annelise E von Bergen Granell; Karen B Palter; Ihan Akan; Udayanath Aich; Kevin J Yarema; Michael J Betenbaugh; William B Thornhill; Esperanza Recio-Pinto
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-09-29
Journal Detail:
Title:  ACS chemical biology     Volume:  6     ISSN:  1554-8937     ISO Abbreviation:  ACS Chem. Biol.     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-18     Completed Date:  2012-05-02     Revised Date:  2014-09-15    
Medline Journal Info:
Nlm Unique ID:  101282906     Medline TA:  ACS Chem Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1287-95     Citation Subset:  IM    
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MeSH Terms
Cells, Cultured
Central Nervous System / cytology*,  growth & development*
Drosophila melanogaster / cytology*,  enzymology,  growth & development,  metabolism*
HEK293 Cells
Larva / cytology,  enzymology,  metabolism
N-Acetylneuraminic Acid / biosynthesis*
Neurons / cytology,  enzymology,  metabolism*
Oxo-Acid-Lyases / metabolism*
Grant Support
Reg. No./Substance:
EC synthase; EC 4.1.3.-/Oxo-Acid-Lyases; GZP2782OP0/N-Acetylneuraminic Acid

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