Document Detail

Diversity within natural populations of eastern equine encephalomyelitis virus.
MedLine Citation:
PMID:  8101674     Owner:  NLM     Status:  MEDLINE    
We evaluated genetic and phenotypic diversity within natural populations of the alphavirus, Eastern equine encephalomyelitis (EEE) virus. RNA fingerprinting revealed that most populations within infected hosts (unpassaged isolates) contained a consensus genotype along with minority genotypes differing in one to three T1-resistant oligonucleotides. Mutation frequencies appeared to be similar to those reported for other RNA viruses, suggesting that the slow rate of EEE virus evolution is not limited by fidelity of genome replication. Within a given year, genetic diversity was generally greater among geographically distant isolates than among those from the same transmission focus, suggesting that dispersal among EEE viruses in North America is not complete annually. Two of three bird isolates from Maryland and New York contained relatively distantly related genotypes, differing in 15-19 oligonucleotides. A 1985 mosquito isolate from Maryland contained stable, small plaque variants which comprised the majority of that population. These small plaque variants differed by up to eight T1-resistant oligonucleotides when compared with their large plaque counterparts. Temperature sensitive virus was not detected in six unpassaged mosquito isolates from Maryland and New York.
S C Weaver; L A Bellew; L Gousset; P M Repik; T W Scott; J J Holland
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Virology     Volume:  195     ISSN:  0042-6822     ISO Abbreviation:  Virology     Publication Date:  1993 Aug 
Date Detail:
Created Date:  1993-08-20     Completed Date:  1993-08-20     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  700-9     Citation Subset:  IM    
Department of Biology, University of California, San Diego, La Jolla 92093.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Cell Line
DNA, Viral
Encephalitis Virus, Eastern Equine / genetics*
Genetic Variation*
Molecular Sequence Data
RNA, Viral
Grant Support
Reg. No./Substance:
0/DNA, Viral; 0/RNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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