Document Detail


Diversity of potential short tandem repeats in Mycobacterium leprae and application for molecular typing.
MedLine Citation:
PMID:  16207987     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A recent advance in molecular typing for tracing the transmission of leprosy is the discovery of short tandem repeats (STRs) in Mycobacterium leprae. To substantiate polymorphic loci from STR as promising candidates for molecular typing tools in leprosy epidemiology, 44 STR loci including 33 microsatellites and 11 minisatellites were investigated among 27 laboratory strains by sequencing PCR products. Not all STRs were necessarily polymorphic. Thirty-two out of the 44 loci were polymorphic. Nine polymorphic loci were suitable for identifying genotypes according to the discriminatory capacity, stability, and reproducibility. All the strains were classified into independent genotypes by the selected nine loci. Three multi-case households were subjected to molecular typing. M. leprae obtained from household cases showed identical copy numbers by TTC triplet alone, but the isolates from one family contact case were divided into different genotypes by adding eight other polymorphic loci. The combination of information from multiple loci allows increasing levels of discrimination and it is likely that the generation and documentation of data will result in the choice of a potential molecular typing tool for leprosy epidemiology.
Authors:
Liangfen Zhang; Teky Budiawan; Masanori Matsuoka
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  43     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-10-06     Completed Date:  2005-11-28     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5221-9     Citation Subset:  IM    
Affiliation:
Leprosy Research Center, National Institute of Infectious Diseases, 4-2-1, Aobacho, Higashimurayama-shi, Tokyo 189-0002, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Typing Techniques*
DNA, Bacterial / analysis
Foot / microbiology
Genetic Markers / genetics*
Genetic Variation*
Humans
Leprosy / epidemiology,  microbiology
Mice
Mice, Inbred BALB C
Mice, Nude
Microsatellite Repeats / genetics
Minisatellite Repeats / genetics
Mycobacterium leprae / classification*,  genetics*
Sequence Analysis, DNA
Tandem Repeat Sequences / genetics*
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/Genetic Markers
Comments/Corrections

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