| Diversity patterns and activity of uncultured marine heterotrophic flagellates unveiled with pyrosequencing. | |
| | |
MedLine Citation:
|
PMID: 22534609 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Flagellated heterotrophic microeukaryotes have key roles for the functioning of marine ecosystems as they channel large amounts of organic carbon to the upper trophic levels and control the population sizes of bacteria and archaea. Still, we know very little on the diversity patterns of most groups constituting this evolutionary heterogeneous assemblage. Here, we investigate 11 groups of uncultured flagellates known as MArine STramenopiles (MASTs). MASTs are ecologically very important and branch at the base of stramenopiles. We explored the diversity patterns of MASTs using pyrosequencing (18S rDNA) in coastal European waters. We found that MAST groups range from highly to lowly diversified. Pyrosequencing (hereafter '454') allowed us to approach to the limits of taxonomic diversity for all MAST groups, which varied in one order of magnitude (tens to hundreds) in terms of operational taxonomic units (98% similarity). We did not evidence large differences in activity, as indicated by ratios of DNA:RNA-reads. Most groups were strictly planktonic, although we found some groups that were active in sediments and even in anoxic waters. The proportion of reads per size fraction indicated that most groups were composed of very small cells (∼2-5 μm). In addition, phylogenetically different assemblages appeared to be present in different size fractions, depths and geographic zones. Thus, MAST diversity seems to be highly partitioned in spatial scales. Altogether, our results shed light on these ecologically very important but poorly known groups of uncultured marine flagellates.The ISME Journal advance online publication, 26 April 2012; doi:10.1038/ismej.2012.36. |
| | |
Authors:
|
Ramiro Logares; Stephane Audic; Sebastien Santini; Massimo C Pernice; Colomban de Vargas; Ramon Massana |
Related Documents
:
|
10633019 - Influence of dietary simulating solvents on the viscoelasticity of temporary soft linin... 22408909 - Chemical functionalization of diatom silica microparticles for adsorption of gold (iii)... 16256589 - Determination of coupled solvent mass in quartz crystal microbalance measurements using... 22513819 - What drives the precipitation of long-chain calcium carboxylates (soaps) in aqueous sol... 21211149 - Lucifer yellow as a live cell fluorescent probe for imaging water transport in subcellu... 10824159 - Unusual solvent effects on chiroptical properties of an optically active regioregular p... 18351589 - Role of structural water molecule in hiv protease-inhibitor complexes: a qm/mm study. 17252999 - Methane oxidation in water-spreading and compost biofilters. 1815359 - Qsar for organic chemical bioconcentration in daphnia, algae, and mussels. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-4-26 |
Journal Detail:
|
Title: The ISME journal Volume: - ISSN: 1751-7370 ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
|
Created Date: 2012-4-26 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101301086 Medline TA: ISME J Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Institut de Ciències del Mar (CSIC), Passeig Marítim de la Barceloneta, Barcelona, Spain. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Comparison of large-insert, small-insert and pyrosequencing libraries for metagenomic analysis.
Next Document: A metaproteomic assessment of winter and summer bacterioplankton from Antarctic Peninsula coastal su...