Document Detail


Diversity and expression of RubisCO genes in a perennially ice-covered Antarctic lake during the polar night transition.
MedLine Citation:
PMID:  22492447     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The autotrophic communities in the lakes of the McMurdo Dry Valleys, Antarctica, have generated interest since the early 1960s owing to low light transmission through the permanent ice covers, a strongly bimodal seasonal light cycle, constant cold water temperatures, and geographical isolation. Previous work has shown that autotrophic carbon fixation in these lakes provides an important source of organic matter to this polar desert. Lake Bonney has two lobes separated by a shallow sill and is one of several chemically stratified lakes in the dry valleys that support year-round biological activity. As part of an International Polar Year initiative, we monitored the diversity and abundance of major isoforms of RubisCO in Lake Bonney by using a combined sequencing and quantitative PCR approach during the transition from summer to polar winter. Form ID RubisCO genes related to a stramenopile, a haptophyte, and a cryptophyte were identified, while primers specific for form IA/B RubisCO detected a diverse autotrophic community of chlorophytes, cyanobacteria, and chemoautotrophic proteobacteria. Form ID RubisCO dominated phytoplankton communities in both lobes of the lake and closely matched depth profiles for photosynthesis and chlorophyll. Our results indicate a coupling between light availability, photosynthesis, and rbcL mRNA levels in deep phytoplankton populations. Regulatory control of rbcL in phytoplankton living in nutrient-deprived shallow depths does not appear to be solely light dependent. The distinct water chemistries of the east and west lobes have resulted in depth- and lobe-dependent variability in RubisCO diversity, which plays a role in transcriptional activity of the key gene responsible for carbon fixation.
Authors:
Weidong Kong; David C Ream; John C Priscu; Rachael M Morgan-Kiss
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-04-06
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  78     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-05-24     Completed Date:  2012-08-29     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4358-66     Citation Subset:  IM    
Affiliation:
Department of Medicine, University of California, San Francisco, California, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/GU132860;  GU132861;  GU132862;  GU132863;  GU132864;  GU132865;  GU132866;  GU132867;  GU132868;  GU132869;  GU132870;  GU132871;  GU132872;  GU132873;  GU132874;  GU132875;  GU132876;  GU132877;  GU132878;  GU132879;  GU132880;  GU132881;  GU132882;  GU132883;  GU132884;  GU132885;  GU132886;  GU132887;  GU132888;  GU132889;  GU132890;  GU132891;  GU132892;  GU132893;  GU132894;  GU132895;  GU132896;  GU132897;  GU132898;  GU132899;  GU132900;  GU132901;  GU132902;  GU132903;  GU132904;  GU132905;  GU132906;  GU132907;  GU132908;  GU132909;  GU132910;  GU132911;  GU132912;  GU132913;  GU132914;  GU132915;  GU132916;  GU132917;  GU132918;  GU132919;  GU132920;  GU132921;  GU132922;  GU132923;  GU132924;  GU132925;  GU132926;  GU132927;  GU132928;  GU132929;  GU132930;  GU132931;  GU132932;  GU132933;  GU132934;  GU132935;  GU132936;  GU132937;  GU132938;  GU132939
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MeSH Terms
Descriptor/Qualifier:
Antarctic Regions
Autotrophic Processes
Chlorophyta / chemistry,  genetics
DNA, Bacterial / chemistry,  genetics
DNA, Fungal / chemistry,  genetics
Genetic Variation*
Molecular Sequence Data
Real-Time Polymerase Chain Reaction
Ribulose-Bisphosphate Carboxylase / biosynthesis*,  genetics*
Seasons
Sequence Analysis, DNA
Water Microbiology*
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/DNA, Fungal; EC 4.1.1.39/Ribulose-Bisphosphate Carboxylase
Comments/Corrections

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