| Diversity and distribution of sulphate-reducing bacteria in human faeces from healthy subjects and patients with inflammatory bowel disease. | |
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MedLine Citation:
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PMID: 22309113 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The relative abundance of different groups of sulphate reducing bacteria (SRB) in faecal DNA collected before and after therapy from patients suffering with Crohn's disease (CD), irritable bowel syndrome (IBS) or ulcerative colitis (UC) has been compared with that from healthy controls. Growth tests revealed that SRB were not more abundant in samples from CD patients before treatment than in the healthy control group. For most of the 128 samples available, these preliminary results were confirmed using degenerate PCR primers that amplify the dsrAB gene. However, some samples from CD patients before treatment contained a growth inhibitor that was absent from IBS or UC samples. In-depth sequencing of PCR-generated dsrB fragments revealed that the diversity detected was surprisingly low, with only 8 strains of SRB and the sulphite reducing bacterium, Bilophila wadsworthia, detected above the 0.1% threshold. The proportion of the two major species detected, B. wadsworthia and Desulfovibrio piger, was as high as 93.5% of the total SRB population in the healthy control group, and lower in all patient groups. Four previously undescribed species were found: it is impossible to predict whether they are sulphate or sulphite reducing bacteria. © 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved. |
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Authors:
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Wenjing Jia; Rebekah N Whitehead; Lesley Griffiths; Claire Dawson; Hao Bai; Rosemary H Waring; David B Ramsden; John O Hunter; Michael Cauchi; Conrad Bessant; Dawn P Fowler; Christopher Walton; Claire Turner; Jeffrey A Cole |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-2-6 |
Journal Detail:
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Title: FEMS immunology and medical microbiology Volume: - ISSN: 1574-695X ISO Abbreviation: - Publication Date: 2012 Feb |
Date Detail:
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Created Date: 2012-2-7 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9315554 Medline TA: FEMS Immunol Med Microbiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved. |
Affiliation:
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School of Biosciences, University of Birmingham, Birmingham, B15 2TT, UK. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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