Document Detail


Diversity of a complex centromeric satellite and molecular characterization of dispersed sequence families in sugar beet (Beta vulgaris).
MedLine Citation:
PMID:  18682437     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND AND AIMS: The aim of this work was the identification and molecular characterization of novel sugar beet (Beta vulgaris) repetitive sequences to unravel the impact of repetitive DNA on size and evolution of Beta genomes via amplification and diversification.
METHODS: Genomic DNA and a pool of B. vulgaris repetitive sequences were separately used as probes for a screening of high-density filters from a B. vulgaris plasmid library. Novel repetitive motifs were identified by sequencing and further used as probes for Southern analyses in the genus Beta. Chromosomal localization of the repeats was analysed by fluorescent in situ hybridization on chromosomes of B. vulgaris and two other species of the section Beta.
KEY RESULTS: Two dispersed repetitive families pDvul1 and pDvul2 and the tandemly arranged repeat family pRv1 were isolated from a sugar beet plasmid library. The dispersed repetitive families pDvul1 and pDvul2 were identified in all four sections of the genus Beta. The members of the pDvul1 and pDvul2 family are scattered over all B. vulgaris chromosomes, although amplified to a different extent. The pRv1 satellite repeat is exclusively present in species of the section Beta. The centromeric satellite pBV1 by structural variations of the monomer and interspersion of pRv1 units forms complex satellite structures, which are amplified in different degrees on the centromeres of 12 chromosomes of the three species of the Beta section.
CONCLUSIONS: The complexity of the pBV1 satellite family observed in the section Beta of the genus Beta and, in particular, the strong amplification of the pBV1/pRv1 satellite in the domesticated B. vulgaris indicates the dynamics of centromeric satellite evolution during species radiation within the genus. The dispersed repeat families pDvul1 and pDvul2 might represent derivatives of transposable elements.
Authors:
Gerhard Menzel; Daryna Dechyeva; Torsten Wenke; Daniela Holtgräwe; Bernd Weisshaar; Thomas Schmidt
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-08-05
Journal Detail:
Title:  Annals of botany     Volume:  102     ISSN:  1095-8290     ISO Abbreviation:  Ann. Bot.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-09-18     Completed Date:  2008-10-20     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  0372347     Medline TA:  Ann Bot     Country:  England    
Other Details:
Languages:  eng     Pagination:  521-30     Citation Subset:  IM    
Affiliation:
Institute of Botany, Dresden University of Technology, D-01062 Dresden, Germany.
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MeSH Terms
Descriptor/Qualifier:
Beta vulgaris / genetics*
Centromere / genetics
Chromosome Banding
Chromosomes, Plant / genetics
DNA, Plant / genetics*
DNA, Satellite / genetics*
Genome, Plant*
Genomic Library
In Situ Hybridization, Fluorescence
Polymerase Chain Reaction
Repetitive Sequences, Nucleic Acid / genetics*
Sequence Analysis, DNA
Species Specificity
Chemical
Reg. No./Substance:
0/DNA, Plant; 0/DNA, Satellite
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