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Diversity of antimicrobial resistance genes and class-1-integrons in phylogenetically related porcine and human Escherichia coli.
MedLine Citation:
PMID:  22854332     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Antimicrobial resistant bacteria and resistance genes can be transferred between the microbial flora of humans and animals. To assess the dimension of this risk, we compared the phylogenetic ancestry of human and porcine tetracycline-insusceptible Escherichia coli. Further, we compared the resistance gene profiles (tetA/tetB/tetC/tetD/tetM/sulI/sulII/sulIII/strA-strB/addA) and the prevalence of class-1-integrons in isolates of identical and different phylogroups by endpoint-PCR. This is the first genotypic comparison of antimicrobial resistance in E. coli from humans and animals which allows for the phylogenetic ancestry of the isolates. E. coli isolates from diseased humans belonged regularly to phylogroup B2 (24.3%) or D (30.9%) and were rarely not typeable (7.2%); by contrast, isolates from pig manure were regularly not typeable (46.7%) and rarely grouped into phylogroup B2 (2.2%) or D (2.9%). Class-1-integrons were detected in 40.8% of clinical (n=152), in 9.5% of community-derived (n=21) and in 10.9% of porcine (n=137) E. coli. The prevalence of sulI (42.4%/16.0%) in phylogroup A and of tetA, tetB and sulII in phylogroup B1 differed significantly between human clinical and porcine strains. Human clinical isolates (except B2-isolates) carried significantly more different resistance genes per strain, compared to porcine or community-derived isolates. ERIC-PCR-analysis of B2- (and D-) isolates with identical genetic profiles revealed that only a minor part was clonally related. The dominant resistance gene profiles differed depending on phylogroup and source. Human and porcine isolates do not exceedingly share their genes, and might rapidly adapt their resistance gene equipment to meet the requirements of a new environment. The study underlines that resistance gene transfer between human and porcine isolates is limited, even in phylogenetically related isolates.
Authors:
Christina Susanne Hölzel; Katrin Susanne Harms; Johann Bauer; Ilse Bauer-Unkauf; Stefan Hörmansdorfer; Peter Kämpf; Gabriele Mölle; Cornelia Oehme; Petra Preikschat; Karin Schwaiger
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-16
Journal Detail:
Title:  Veterinary microbiology     Volume:  -     ISSN:  1873-2542     ISO Abbreviation:  Vet. Microbiol.     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-8-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7705469     Medline TA:  Vet Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
Affiliation:
Technische Universität München, Weihenstephaner Berg 3, 85354 Freising, Germany.
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