Document Detail


Diversity of T-cell antigen receptor V beta gene utilization in advanced human atheroma.
MedLine Citation:
PMID:  8018677     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human atheromata contain T lymphocytes, but knowledge of the function and receptor specificity of these cells is limited. Immunohistochemical studies have established that T cells in advanced human carotid plaques express predominantly the alpha/beta form of the T-cell receptor (TCR). We then compared the use of variable region genes of the beta-chain (V beta) of the TCR for antigen by analysis of 14 carotid plaques and peripheral blood samples obtained at carotid endarterectomy. We used a direct approach that avoids isolation and culture of T cells. RNA extracted from lesions and peripheral blood mononuclear cells was reverse transcribed and amplified by polymerase chain reaction (PCR) to determine rearrangements of 18 V beta sequences. PCR products were visualized on Southern blots using a probe internal to the PCR primers. Input cDNA from lesions and peripheral blood was adjusted to yield equivalent signals for a conserved region of the TCR beta-chain to permit comparisons. As expected, utilization of TCR V beta genes in peripheral blood cells was nonselective: an average of 17 of 18 V beta regions yielded signals (n = 14). Frequency of variable-region gene usage in lesions and blood was highly concordant: of 252 sequences tested (14 samples, 18 sequences per sample), 240 were identified in peripheral blood versus 207 in plaques. V beta genes 10 and 11 were not expressed in plaques, a significant difference when compared with peripheral blood (P = .0001 by chi 2). However, the remaining 16 genes showed no significant differences. This analysis indicates that T cells generally express a diverse pattern of V beta genes within complex human atheroma.
Authors:
S J Swanson; A Rosenzweig; J G Seidman; P Libby
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Arteriosclerosis and thrombosis : a journal of vascular biology / American Heart Association     Volume:  14     ISSN:  1049-8834     ISO Abbreviation:  Arterioscler. Thromb.     Publication Date:  1994 Jul 
Date Detail:
Created Date:  1994-08-03     Completed Date:  1994-08-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9101388     Medline TA:  Arterioscler Thromb     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1210-4     Citation Subset:  IM    
Affiliation:
Department of Medicine, Harvard Medical School, Boston, Mass. 02115.
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MeSH Terms
Descriptor/Qualifier:
Arteriosclerosis / immunology*
Blotting, Southern
Humans
Immunoenzyme Techniques
Immunoglobulin Variable Region / genetics*
Polymerase Chain Reaction
Receptors, Antigen, T-Cell, alpha-beta / genetics*
Receptors, Antigen, T-Cell, gamma-delta / immunology
T-Lymphocytes / immunology*
Grant Support
ID/Acronym/Agency:
HL-43364/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Immunoglobulin Variable Region; 0/Receptors, Antigen, T-Cell, alpha-beta; 0/Receptors, Antigen, T-Cell, gamma-delta

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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