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Diversity of the Luciferin Binding Protein Gene in Bioluminescent Dinoflagellates - Insights from a New Gene in Noctiluca scintillans and Sequences from Gonyaulacoid Genera.
MedLine Citation:
PMID:  24373055     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Dinoflagellate bioluminescence systems operate with or without a luciferin binding protein, representing two distinct modes of light production. However, the distribution, diversity, and evolution of the luciferin binding protein gene within bioluminescent dinoflagellates are not well known. We used PCR to detect and partially sequence this gene from the heterotrophic dinoflagellate Noctiluca scintillans and a group of ecologically important gonyaulacoid species. We report an additional luciferin binding protein gene in N. scintillans which is not attached to luciferase, further to its typical combined bioluminescence gene. This supports the hypothesis that a profound re-organization of the bioluminescence system has taken place in this organism. We also show that the luciferin binding protein gene is present in the genera Ceratocorys, Gonyaulax, and Protoceratium, and is prevalent in bioluminescent species of Alexandrium. Therefore, this gene is an integral component of the standard molecular bioluminescence machinery in dinoflagellates. Nucleotide sequences showed high within-strain variation among gene copies, revealing a highly diverse gene family comprising multiple gene types in some organisms. Phylogenetic analyses showed that, in some species, the evolution of the luciferin binding protein gene was different from the organism's general phylogenies, highlighting the complex evolutionary history of dinoflagellate bioluminescence systems.
Authors:
Martha Valiadi; Maria Debora Iglesias-Rodriguez
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-11-8
Journal Detail:
Title:  The Journal of eukaryotic microbiology     Volume:  -     ISSN:  1550-7408     ISO Abbreviation:  J. Eukaryot. Microbiol.     Publication Date:  2013 Nov 
Date Detail:
Created Date:  2013-12-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9306405     Medline TA:  J Eukaryot Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 The Author(s) Journal of Eukaryotic Microbiology © 2013 International Society of Protistologists.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/EU236684;  JF838193;  JQ946837;  JQ946839;  JQ946841;  JQ946843;  JQ946845;  JQ946854;  JQ946863;  JQ946865;  KF601764;  JQ946867
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