Document Detail

Diversity of Kenyan soda lake alkaliphiles assessed by molecular methods.
MedLine Citation:
PMID:  15064991     Owner:  NLM     Status:  MEDLINE    
DNA was extracted from water and sediment samples taken from soda lakes of the Kenyan-Tanzanian Rift Valley. DNA was also extracted from microbial enrichment cultures of sediment samples. 16S rRNA genes were amplified by the polymerase chain reaction and microbial diversity was studied using denaturing gradient gel electrophoresis (DGGE) of 16S rDNA amplicons. Cloning and sequencing of single DGGE bands showed that they usually contained mixed amplicons. Several of the amplicon sequences had high identities, up to 99%, with 16S rRNA genes of organisms previously isolated from soda lakes, while others were only distantly related, with identities as low as 82%. Phylogenetic analysis of the sequenced amplicons indicated that sequences were related to the haloarchaeal, Bacillus/Clostridium, Rhodobacterium/ Thioalcalovibrio/ Methylobacter, and Cytophaga/Flavobacterium/Bacteroides (CFB) groups and the enterobacteria/ Aeromonas/Vibrio part of the gamma3 subdivision of the Proteobacteria.
Helen C Rees; William D Grant; Brian E Jones; Shaun Heaphy
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-10-29
Journal Detail:
Title:  Extremophiles : life under extreme conditions     Volume:  8     ISSN:  1431-0651     ISO Abbreviation:  Extremophiles     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-04-05     Completed Date:  2004-10-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9706854     Medline TA:  Extremophiles     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  63-71     Citation Subset:  IM; S    
Department of Microbiology and Immunology, University of Leicester, University Road, LE1 9HN, Leicester, UK.
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MeSH Terms
Archaea / classification,  genetics*,  isolation & purification*
Bacteria / classification,  genetics*,  isolation & purification*
DNA, Archaeal / genetics,  isolation & purification
DNA, Bacterial / genetics,  isolation & purification
Fresh Water / microbiology*
Hydrogen-Ion Concentration
RNA, Archaeal / genetics
RNA, Bacterial / genetics
RNA, Ribosomal, 16S / genetics
Reg. No./Substance:
0/Carbonates; 0/DNA, Archaeal; 0/DNA, Bacterial; 0/RNA, Archaeal; 0/RNA, Bacterial; 0/RNA, Ribosomal, 16S; 497-19-8/sodium carbonate

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