| Diversity of Cultivable Methane-Oxidizing Bacteria in Microsites of a Rice Paddy Field: Investigation by Cultivation Method and Fluorescence in situ Hybridization (FISH). | |
| | |
MedLine Citation:
|
PMID: 22446309 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
The diversity of cultivable methane-oxidizing bacteria (MOB) in the rice paddy field ecosystem was investigated by combined culture-dependent and fluorescence in situ hybridization (FISH) techniques. Seven microsites of a Japanese rice paddy field were the focus of the study: floodwater, surface soil, bulk soil, rhizosphere soil, root, basal stem of rice plant, and rice stumps of previous harvest. Based on pmoA gene analysis and transmission electron microscopy (TEM), four type I, and nine type II MOB isolates were obtained from the highest dilution series of enrichment cultures. The type I MOB isolates included a novel species in the genus Methylomonas from floodwater and this is the first type I MOB strain isolated from floodwater of a rice paddy field. In the type I MOB, two isolates from stumps were closely related to Methylomonas spp.; one isolate obtained from rhizosphere soil was most related to Methyloccocus-Methylocaldum-Methylogaea clade. Almost all the type II MOB isolates were related to Methylocystis methanotrophs. FISH confirmed the presence of both types I and II MOB in all the microsites and in the related enrichment cultures. The study reported, for the first time, the diversity of cultivable methanotrophs including a novel species of type I MOB in rice paddy field compartments. Refining growth media and culture conditions, in combination with molecular approaches, will allow us to broaden our knowledge on the MOB community in the rice paddy field ecosystem and consequently to implement strategies for mitigating CH4 emission from this ecosystem. |
| | |
Authors:
|
Dayéri Dianou; Chihoko Ueno; Takuya Ogiso; Makoto Kimura; Susumu Asakawa |
Related Documents
:
|
8756589 - Molecular characterisation of an s-like rnase of nicotiana alata that is induced by pho... 20400679 - Rna-dependent rna polymerase 1 from nicotiana tabacum suppresses rna silencing and enha... 20331529 - Tapping rna silencing pathways for plant biotechnology. 22819889 - Has alberta oil sands development increased far-field delivery of airborne contaminants... 22555519 - Effects silver nanoparticles and magnetic field on growth of fodder maize (zea mays l.). 22420269 - Field attraction of the vine weevil otiorhynchus sulcatus to kairomones. 11240069 - Impact of thermal treatment on metal in sewage sludge from the psittalias wastewater tr... 12425959 - Assessment of the potential genotoxic risk of phyllantus orbicularis hbk aqueous extrac... 21672619 - Suitability of non-lethal marker and marker-free systems for development of transgenic ... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-3-23 |
Journal Detail:
|
Title: Microbes and environments / JSME Volume: - ISSN: 1347-4405 ISO Abbreviation: - Publication Date: 2012 Mar |
Date Detail:
|
Created Date: 2012-3-26 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9710937 Medline TA: Microbes Environ Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Centre National de la recherche Scientifique et technologique. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Isolation and Characterization of Novel Sulfate-Reducing Bacterium Capable of Anaerobic Degradation ...
Next Document: Novel Conjugative Transferable Multiple Drug Resistance Plasmid pAQU1 from Photobacterium damselae s...