| Diverse roles for protein kinase C delta and protein kinase C epsilon in the generation of high-fat-diet-induced glucose intolerance in mice: regulation of lipogenesis by protein kinase C delta. | |
| | |
MedLine Citation:
|
PMID: 19809797 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
AIMS/HYPOTHESIS: This study aimed to determine whether protein kinase C (PKC) delta plays a role in the glucose intolerance caused by a high-fat diet, and whether it could compensate for loss of PKCepsilon in the generation of insulin resistance in skeletal muscle. METHODS: Prkcd (-/-), Prkce (-/-) and wild-type mice were fed high-fat diets and subjected to glucose tolerance tests. Blood glucose levels and insulin responses were determined during the tests. Insulin signalling in liver and muscle was assessed after acute in vivo insulin stimulation by immunoblotting with phospho-specific antibodies. Activation of PKC isoforms in muscle from Prkce (-/-) mice was assessed by determining intracellular distribution. Tissues and plasma were assayed for triacylglycerol accumulation, and hepatic production of lipogenic enzymes was determined by immunoblotting. RESULTS: Both Prkcd (-/-) and Prkce (-/-) mice were protected against high-fat-diet-induced glucose intolerance. In Prkce (-/-) mice this was mediated through enhanced insulin availability, while in Prkcd (-/-) mice the reversal occurred in the absence of elevated insulin. Neither the high-fat diet nor Prkcd deletion affected maximal insulin signalling. The activation of PKCdelta in muscle from fat-fed mice was enhanced by Prkce deletion. PKCdelta-deficient mice exhibited reduced liver triacylglycerol accumulation and diminished production of lipogenic enzymes. CONCLUSIONS/INTERPRETATION: Deletion of genes encoding isoforms of PKC can improve glucose intolerance, either by enhancing insulin availability in the case of Prkce, or by reducing lipid accumulation in the case of Prkcd. The absence of PKCepsilon in muscle may be compensated by increased activation of PKCdelta in fat-fed mice, suggesting that an additional role for PKCepsilon in this tissue is masked. |
| | |
Authors:
|
G Frangioudakis; J G Burchfield; S Narasimhan; G J Cooney; M Leitges; T J Biden; C Schmitz-Peiffer |
Related Documents
:
|
19698707 - Role of nrf2 in prevention of high-fat diet-induced obesity by synthetic triterpenoid c... 18812577 - Influence of helicobacter hepaticus infection on the chronic toxicity and carcinogenici... 2957297 - Adrenalectomy and steroid treatment in obese (ob/ob) and diabetic (db/db) mice. 11929197 - Docosahexaenoic acid-rich phospholipid supplementation: effect on behavior, learning ab... 12858127 - Emerging importance of hdl cholesterol in developing high-risk coronary plaques in acut... 3214137 - Seasonal variations of serum lipids and apoproteins. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-10-07 |
Journal Detail:
|
Title: Diabetologia Volume: 52 ISSN: 1432-0428 ISO Abbreviation: Diabetologia Publication Date: 2009 Dec |
Date Detail:
|
Created Date: 2010-01-21 Completed Date: 2010-04-23 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0006777 Medline TA: Diabetologia Country: Germany |
Other Details:
|
Languages: eng Pagination: 2616-20 Citation Subset: IM |
Affiliation:
|
Diabetes and Obesity Program, Garvan Institute of Medical Research, 384 Victoria Street, Darlinghurst, New South Wales, 2010, Australia. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Blood Glucose / metabolism Crosses, Genetic Dietary Fats / adverse effects* Gene Deletion Glucose Intolerance / blood, chemically induced*, enzymology Mice Mice, Inbred C57BL Mice, Inbred Strains Mice, Knockout Muscle, Skeletal / enzymology Protein Kinase C-delta / deficiency*, genetics, metabolism* Protein Kinase C-epsilon / deficiency*, genetics, metabolism* Triglycerides / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Blood Glucose; 0/Dietary Fats; 0/Triglycerides; EC 2.7.11.13/Protein Kinase C-delta; EC 2.7.11.13/Protein Kinase C-epsilon |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Polymorphisms within insulin-degrading enzyme (IDE) gene determine insulin metabolism and risk of ty...
Next Document: Insights into the critical role of NADPH oxidase(s) in the normal and dysregulated pancreatic beta c...