| Diverse roles of hnRNP L in mammalian mRNA processing: a combined microarray and RNAi analysis. | |
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MedLine Citation:
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PMID: 18073345 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Alternative mRNA splicing patterns are determined by the combinatorial control of regulator proteins and their target RNA sequences. We have recently characterized human hnRNP L as a global regulator of alternative splicing, binding to diverse C/A-rich elements. To systematically identify hnRNP L target genes on a genome-wide level, we have combined splice-sensitive microarray analysis and an RNAi-knockdown approach. As a result, we describe 11 target genes of hnRNP L that were validated by RT-PCR and that represent several new modes of hnRNP L-dependent splicing regulation, involving both activator and repressor functions: first, intron retention; second, inclusion or skipping of cassette-type exons; third, suppression of multiple exons; and fourth, alternative poly(A) site selection. In sum, this approach revealed a surprising diversity of splicing-regulatory processes as well as poly(A) site selection in which hnRNP L is involved. |
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Authors:
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Lee-Hsueh Hung; Monika Heiner; Jingyi Hui; Silke Schreiner; Vladimir Benes; Albrecht Bindereif |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-12-11 |
Journal Detail:
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Title: RNA (New York, N.Y.) Volume: 14 ISSN: 1469-9001 ISO Abbreviation: RNA Publication Date: 2008 Feb |
Date Detail:
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Created Date: 2008-01-25 Completed Date: 2008-03-07 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 9509184 Medline TA: RNA Country: United States |
Other Details:
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Languages: eng Pagination: 284-96 Citation Subset: IM |
Affiliation:
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Institute of Biochemistry, Justus-Liebig-University of Giessen, D-35392 Giessen, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alternative Splicing
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genetics* Amino Acid Motifs Exons Hela Cells Heterogeneous-Nuclear Ribonucleoprotein L / antagonists & inhibitors, genetics, physiology* Humans Introns Oligonucleotide Array Sequence Analysis / methods* Polyadenylation RNA Interference RNA, Messenger / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Heterogeneous-Nuclear Ribonucleoprotein L; 0/RNA, Messenger |
| Comments/Corrections | |
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