Document Detail


Diverse prevalence of 16S rRNA methylase genes armA and rmtB amongst clinical multidrug-resistant Escherichia coli and Klebsiella pneumoniae isolates.
MedLine Citation:
PMID:  21724374     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
In this study, 112 Escherichia coli and 55 Klebsiella pneumoniae isolates with a multidrug-resistant (MDR) phenotype were collected from 2007 to 2009. All isolates simultaneously exhibited resistance to cefotaxime (or ceftazidime), ciprofloxacin (or levofloxacin) and amikacin. Plasmid-mediated 16S rRNA methylases, including armA, rmtA, rmtB, rmtC, rmtD, rmtE and npmA, were detected by polymerase chain reaction (PCR) amplification. Common β-lactamase genes, including bla(TEM), bla(SHV), bla(CTX-M), bla(PER), bla(VEB), bla(GES) and bla(OXA), as well as plasmid-mediated bla(AmpC) and plasmid-mediated quinolone resistance (PMQR) determinants, including qnrA, qnrB, qnrS, qepA and aac(6')-Ib-cr, were also screened. The transferable capacity of resistance plasmids was established by conjugation testing. The genetic relatedness of isolates was analysed by pulsed-field gel electrophoresis (PFGE). Only armA and rmtB genes were detected in this study. Data showed that 93.8% of MDR E. coli and 94.5% of MDR K. pneumoniae carried at least one of armA or rmtB. The armA and rmtB genes were present in 11.6% and 82.1% of MDR E. coli, respectively. In parallel, 58.2% and 40.0% of MDR K. pneumoniae were armA- and rmtB-positive, respectively. Furthermore, the qepA gene was present in 66.3% of rmtB-carrying MDR E. coli, but it was rarely detected in MDR K. pneumoniae. Approximately 71.9% of armA-positive MDR K. pneumoniae simultaneously co-carried qnrB and bla(DHA). Moreover, 78.1% and 63.6%, respectively, of armA-positive and rmtB-positive MDR K. pneumoniae strains harboured qnr alleles and 53.1% and 59.1% harboured aac(6')-Ib-cr. In addition, MDR E. coli strains exhibited a low prevalence of qnr alleles and aac(6')-Ib-cr. PFGE analysis revealed divergent genetic relatedness, suggesting horizontal dissemination of armA and rmtB along with common β-lactamases and PMQR determinants amongst clinical MDR E. coli and K. pneumoniae isolates.
Authors:
Jiyong Yang; Liyan Ye; Weiwei Wang; Yanping Luo; Youjiang Zhang; Li Han
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-7-1
Journal Detail:
Title:  International journal of antimicrobial agents     Volume:  -     ISSN:  1872-7913     ISO Abbreviation:  -     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-7-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9111860     Medline TA:  Int J Antimicrob Agents     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
Affiliation:
Department of Microbiology, Chinese PLA General Hospital, Beijing, China.
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