Document Detail


Diverse expression of delayed rectifier K+ channel subtype transcripts in several types of smooth muscles of the rat.
MedLine Citation:
PMID:  11086882     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Diverse expression of voltage dependent K+ (Kv) channels was examined in smooth muscles (SMs); carotid artery (CA), mesenteric artery (MA), urinary bladder (UB), and vas deferens (VD) of the rat, using RT-PCR based analyses. Among eight Kv channel subtypes examined (Kv 1.1, Kv 1.2, Kv 1.5, Kv 1.6, Kv 2.1, Kv 2.2, Kv 3.1, and Kv 3.2), expression of three delayed rectifier Kv (KD) channel (Kv 1.2, Kv 1.5, and Kv 2.1) transcripts was observed in these SMs. To determine precisely the expression levels of the transcripts encoding K(D) subtypes, those of three K(D) subtypes (Kv 1.2, Kv 1.5, and Kv 2.1) were determined by competitive PCR. In vascular SM tissues, CA and MA, Kv 1.2 and Kv 1.5 transcripts were expressed at relatively high levels, whereas in visceral SM tissues, UB and VD, Kv 2.1 transcripts were expressed at the relatively high levels. These results suggest that the diverse expression of K(D) subtypes is, at least in part, responsible for differences in electrical excitability and also for the variation of the electrophysiological and pharmacological phenotypes as tonic and phasic SMs.
Authors:
S Ohya; M Tanaka; M Watanabe; Y Maizumi
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi     Volume:  36     ISSN:  0916-8737     ISO Abbreviation:  J Smooth Muscle Res     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2001-03-06     Completed Date:  2001-05-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9211664     Medline TA:  J Smooth Muscle Res     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  101-15     Citation Subset:  IM    
Affiliation:
Department of Molecular and Cellular Pharmacology, Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carotid Arteries / physiology
Electrophysiology
Male
Mesenteric Arteries / physiology
Muscle Contraction / physiology
Muscle, Smooth / physiology*
Muscle, Smooth, Vascular / physiology
Potassium Channels / physiology*
Rats
Rats, Wistar
Urinary Bladder / physiology
Vas Deferens / physiology
Chemical
Reg. No./Substance:
0/Potassium Channels

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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