Document Detail

Diverse contribution of bone marrow-derived cells to vascular remodeling associated with pulmonary arterial hypertension and arterial neointimal formation.
MedLine Citation:
PMID:  17242277     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Recent evidence suggests that bone marrow (BM)-derived cells may differentiate into vascular cells that participate in arterial repair and/or lesion formation. However, it remains uncertain whether BM-derived cells also can participate in vascular remodeling associated with pulmonary arterial hypertension. METHODS AND RESULTS: The BM of Sprague-Dawley rats was reconstituted with that of green fluorescent protein-transgenic rats. The BM-chimeric rats were injected intraperitoneally with 60 mg/kg monocrotaline after unilateral subpneumonectomy, and they concurrently underwent wire-mediated endovascular injury in femoral artery. After 28 days, they had elevated right ventricular systolic pressure (58.8+/-5.4 versus 20.4+/-2.4 mm Hg in sham-control; P<0.01). The pulmonary arterioles were markedly thickened, with an infiltration of green fluorescent protein-positive macrophages into the perivascular areas. The endothelium of pulmonary arterioles contained only a few green fluorescent protein-positive cells, and green fluorescent protein-positive cells were seldom detected as smooth muscle cells in the lesions of thickened pulmonary arterioles. In contrast, BM-derived smooth muscle-like cells could be readily detected in the thickened neointima and media of the wire-injured femoral artery. Moreover, intravenous injection of 1x10(8) BM cells from young rats had no beneficial effects on pulmonary hypertension, pulmonary arterial remodeling, or survival in the aged rats treated with monocrotaline plus unilateral subpneumonectomy. No injected BM cell was identified as an endothelial cell or a smooth muscle cell. CONCLUSIONS: These results suggest that BM-derived cells can participate in arterial neointimal formation after mechanical injury, whereas they do not contribute substantially to pulmonary arterial remodeling associated with monocrotaline-induced pulmonary arterial hypertension in the pneumonectomized rats.
Makoto Sahara; Masataka Sata; Toshihiro Morita; Kazuto Nakamura; Yasunobu Hirata; Ryozo Nagai
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-01-22
Journal Detail:
Title:  Circulation     Volume:  115     ISSN:  1524-4539     ISO Abbreviation:  Circulation     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2007-01-30     Completed Date:  2007-02-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0147763     Medline TA:  Circulation     Country:  United States    
Other Details:
Languages:  eng     Pagination:  509-17     Citation Subset:  AIM; IM    
Department of Cardiovascular Medicine, University of Tokyo Graduate School of Medicine, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
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MeSH Terms
Animals, Genetically Modified
Arterioles / pathology
Bone Marrow Cells / pathology*,  physiology*
Bone Marrow Transplantation
Capillaries / pathology
Cell Differentiation
Disease Models, Animal
Femoral Artery / injuries,  pathology
Green Fluorescent Proteins / genetics
Hypertension, Pulmonary / pathology*,  therapy
Monocrotaline / pharmacology
Pulmonary Artery / pathology*
Pulmonary Embolism / pathology
Rats, Sprague-Dawley
Thrombosis / pathology
Tunica Intima / pathology
Ventricular Dysfunction, Right / pathology
Reg. No./Substance:
147336-22-9/Green Fluorescent Proteins; 315-22-0/Monocrotaline

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