Document Detail


Divergent requirements for fibroblast growth factor signaling in zebrafish maxillary barbel and caudal fin regeneration.
MedLine Citation:
PMID:  23350700     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The zebrafish maxillary barbel is an integumentary organ containing skin, glands, pigment cells, taste buds, nerves, and endothelial vessels. The maxillary barbel can regenerate (LeClair & Topczewski 2010); however, little is known about its molecular regulation. We have studied fibroblast growth factor (FGF) pathway molecules during barbel regeneration, comparing this system to a well-known regenerating appendage, the zebrafish caudal fin. Multiple FGF ligands (fgf20a, fgf24), receptors (fgfr1-4) and downstream targets (pea3, il17d) are expressed in normal and regenerating barbel tissue, confirming FGF activation. To test if specific FGF pathways were required for barbel regeneration, we performed simultaneous barbel and caudal fin amputations in two temperature-dependent zebrafish lines. Zebrafish homozygous for a point mutation in fgf20a, a factor essential for caudal fin blastema formation, regrew maxillary barbels normally, indicating that the requirement for this ligand is appendage-specific. Global overexpression of a dominant negative FGF receptor, Tg(hsp70l:dn-fgfr1:EGFP)(pd1) completely blocked fin outgrowth but only partially inhibited barbel outgrowth, suggesting reduced requirements for FGFs in barbel tissue. Maxillary barbels expressing dn-fgfr1 regenerated peripheral nerves, dermal connective tissue, endothelial tubes, and a glandular epithelium; in contrast to a recent report in which dn-fgfr1 overexpression blocks pharyngeal taste bud formation in zebrafish larvae (Kapsimali et al. 2011), we observed robust formation of calretinin-positive tastebuds. These are the first experiments to explore the molecular mechanisms of maxillary barbel regeneration. Our results suggest heterogeneous requirements for FGF signaling in the regeneration of different zebrafish appendages (caudal fin versus maxillary barbel) and taste buds of different embryonic origin (pharyngeal endoderm versus barbel ectoderm).
Authors:
Robert J Duszynski; Jacek Topczewski; Elizabeth E LeClair
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-01-28
Journal Detail:
Title:  Development, growth & differentiation     Volume:  55     ISSN:  1440-169X     ISO Abbreviation:  Dev. Growth Differ.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-02-06     Completed Date:  2013-07-25     Revised Date:  2014-02-04    
Medline Journal Info:
Nlm Unique ID:  0356504     Medline TA:  Dev Growth Differ     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  282-300     Citation Subset:  IM    
Copyright Information:
© 2013 The Authors Development, Growth & Differentiation © 2013 Japanese Society of Developmental Biologists.
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MeSH Terms
Descriptor/Qualifier:
Amputation
Animal Fins / metabolism,  physiology*,  surgery
Animal Structures / metabolism,  physiology*,  surgery
Animals
Animals, Genetically Modified
Female
Fibroblast Growth Factors / genetics,  physiology*
Gene Expression Regulation, Developmental
Green Fluorescent Proteins / genetics,  metabolism
Male
Microscopy, Fluorescence
Mutation
Receptor, Fibroblast Growth Factor, Type 1 / genetics,  metabolism,  physiology
Receptors, Fibroblast Growth Factor / genetics,  metabolism,  physiology*
Regeneration / genetics,  physiology*
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction / genetics,  physiology
Temperature
Zebrafish / genetics,  growth & development,  physiology
Zebrafish Proteins / genetics,  physiology*
Grant Support
ID/Acronym/Agency:
P40-RR012546/RR/NCRR NIH HHS; R01DE016678/DE/NIDCR NIH HHS; R15 HD064169/HD/NICHD NIH HHS; R15HD064169/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Receptors, Fibroblast Growth Factor; 0/Zebrafish Proteins; 0/fibroblast growth factor 20, zebrafish; 0/fibroblast growth factor 24, zebrafish; 147336-22-9/Green Fluorescent Proteins; 62031-54-3/Fibroblast Growth Factors; EC 2.7.10.1/Receptor, Fibroblast Growth Factor, Type 1
Comments/Corrections

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