Document Detail


Divergent lymphocyte signalling revealed by a powerful new tool for analysis of time-lapse microscopy.
MedLine Citation:
PMID:  23032369     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
We describe a new approach for interactive analysis of time-lapse microscopy, and apply this approach to elucidating whether polarity regulation is conserved between epithelial cells and lymphocytes. A key advantage of our analysis platform, 'TACTICS', is the capacity to visualize individual data points in the context of large data sets, similar to standard approaches in flow cytometry. Scatter plots representing microscopic parameters or their derivations such as polarity ratios are linked to the original data such that clicking on each dot enables a link to images and movies of the corresponding cell. Similar to flow cytometric analysis, subsets of the data can be gated and reanalyzed to explore the relationships between different parameters. TACTICS was used to dissect the regulation of polarization of the cell fate determinant, Numb, in migrating lymphocytes. We show here that residues of Numb that are phosphorylated by atypical protein kinase C (aPKC) to mediate apicobasal polarity in epithelial cells are not required for polarization of Numb in T cells, indicating that the role of aPKC is not conserved between lymphocytes and epithelia.Immunology and Cell Biology advance online publication, 2 October 2012; doi:10.1038/icb.2012.49.
Authors:
Kim Pham; Raz Shimoni; Mandy J Ludford-Menting; Cameron J Nowell; Pavel Lobachevsky; Ze'ev Bomzon; Min Gu; Terence P Speed; C Jane McGlade; Sarah M Russell
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-02
Journal Detail:
Title:  Immunology and cell biology     Volume:  -     ISSN:  1440-1711     ISO Abbreviation:  Immunol. Cell Biol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8706300     Medline TA:  Immunol Cell Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
1] Centre for Micro-Photonics, Faculty of Engineering and Industrial Sciences, Swinburne University of Technology, Hawthorn, Adelaide, Australia [2] Immune Signalling Laboratory, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia.
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