Document Detail


Divergent localization of angiotensinogen mRNA and protein in proximal tubule segments of normal rat kidney.
MedLine Citation:
PMID:  23032142     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: Angiotensinogen in the kidneys is formed primarily in the proximal tubule cells and is secreted into the tubular fluid. Structurally, proximal tubules can be divided into three segments. The first segment, segment 1 (S1) is mainly confined to the pars convoluta, the second segment, segment 2 (S2) comprises the end of pars convoluta, and the third segment, segment 3 (S3) includes the major part of the pars recta. There are some reports describing angiotensinogen localization in kidneys; however, it remains uncertain which proximal tubule segments express angiotensinogen. To determine the detailed localization of angiotensinogen in the three proximal tubule segments, we established multistaining methods using segment-specific protein markers.
METHODS: Using kidneys from Wistar-Kyoto rats, we performed immunohistochemistry and double or triple staining by fluorescence in-situ hybridization and/or immunofluorescence.
RESULTS: Our results show that angiotensinogen mRNA and protein are expressed in the cortex and outer medulla of the normal rat kidney. Angiotensinogen mRNA was hardly detected in S1, detected weakly in S2 and strongly in S3 segments. In contrast, angiotensinogen protein was detected in S1 at high levels and less in S2 and S3 segments.
CONCLUSION: These data indicate divergence of angiotensinogen mRNA transcription and angiotensinogen protein synthesis and metabolism in different segments of the normal rat proximal tubules.
Authors:
Masumi Kamiyama; Kristina M Farragut; Michelle K Garner; L Gabriel Navar; Hiroyuki Kobori
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Journal of hypertension     Volume:  30     ISSN:  1473-5598     ISO Abbreviation:  J. Hypertens.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-16     Completed Date:  2013-07-01     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  8306882     Medline TA:  J Hypertens     Country:  England    
Other Details:
Languages:  eng     Pagination:  2365-72     Citation Subset:  IM    
Affiliation:
Department of Physiology, Hypertension and Renal Center of Excellence, Tulane University Health Sciences Center, New Orleans, Louisiana, USA.
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MeSH Terms
Descriptor/Qualifier:
Angiotensinogen / metabolism*
Animals
Biological Markers / metabolism
Fluorescent Antibody Technique
Kidney Cortex / cytology,  metabolism
Kidney Medulla / cytology,  metabolism
Kidney Tubules, Proximal / cytology,  metabolism*
Male
RNA, Messenger / metabolism*
Rats
Rats, Inbred WKY
Grant Support
ID/Acronym/Agency:
P20RR017659/RR/NCRR NIH HHS; R01 DK072408/DK/NIDDK NIH HHS; R01DK072408/DK/NIDDK NIH HHS; R01HL026371/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Biological Markers; 0/RNA, Messenger; 11002-13-4/Angiotensinogen
Comments/Corrections

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