| Divergent effects of cations on follicle-stimulating hormone- and forskolin-activated adenylyl cyclase in granulosa cells. | |
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MedLine Citation:
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PMID: 3109507 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The divalent cations magnesium, calcium and manganese, and the monovalent cation, potassium, but not sodium, enhance binding of [125I]iodo-porcine follicle-stimulating hormone to follicle-stimulating hormone (FSH) receptors in membranes of porcine granulosa cells via an increase in the apparent number of binding sites. The objective of the present studies was to determine if increased binding of FSH to its receptor causes increased adenylyl cyclase activity in response to FSH, or conversely, if enhancement of the cyclase or one of its components causes increased binding, or if the two processes are modulated independently. MgCl2 and CaCl2, which both enhance binding in intact cells and in cell-free membrane preparations, had opposite effects on cyclase-MgCl2 stimulatory, CaCl2 inhibitory. In isotonic NaCl, MgCl2 did not enhance binding, but it did increase FSH-stimulated production of cyclic adenosine 3',5'-monophosphate (cAMP). NaCl did not enhance FSH binding and it did not enhance cyclase in cell-free membranes, but it did increase FSH- and forskolin-stimulated cAMP production in intact cells. In intact cells, maximally effective concentrations of MgCl2 and KCl were additive in enhancing cAMP production whereas the effects of NaCl and KCl together were synergistic. The results indicate that although cationic effects on FSH binding are not causally related to effects on cyclase, the cationic microenvironment of the granulosa cell membrane is critical to both FSH receptor and adenylyl cyclase functions. |
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Authors:
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K A Ford; M Hunzicker-Dunn; A R LaBarbera |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biology of reproduction Volume: 36 ISSN: 0006-3363 ISO Abbreviation: Biol. Reprod. Publication Date: 1987 Apr |
Date Detail:
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Created Date: 1987-07-24 Completed Date: 1987-07-24 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0207224 Medline TA: Biol Reprod Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 651-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenylate Cyclase
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metabolism* Animals Calcium / pharmacology Enzyme Activation / drug effects Female Follicle Stimulating Hormone / pharmacology* Forskolin / pharmacology* Granulosa Cells / drug effects*, metabolism Magnesium / pharmacology Potassium / pharmacology Receptors, FSH / drug effects, metabolism Sodium / pharmacology Swine |
| Grant Support | |
ID/Acronym/Agency:
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HD-11021/HD/NICHD NIH HHS; HD-11356/HD/NICHD NIH HHS; HD-16912/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Receptors, FSH; 66428-89-5/Forskolin; 7439-95-4/Magnesium; 7440-09-7/Potassium; 7440-23-5/Sodium; 7440-70-2/Calcium; 9002-68-0/Follicle Stimulating Hormone; EC 4.6.1.1/Adenylate Cyclase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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