Document Detail

Divergent effects of acute depolarization on somatostatin release and protein synthesis in cultured fetal and neonatal rat brain cells.
MedLine Citation:
PMID:  2468735     Owner:  NLM     Status:  MEDLINE    
The influence of membrane depolarization on somatostatin secretion and protein synthesis by fetal and neonatal cerebrocortical neurons was studied. Cortical cells obtained by mechanical dispersion were maintained as monolayer cultures for 8 days. The ability of fetal cerebrocortical and hypothalamic cells to release immunoreactive somatostatin (IR-SRIF) was confirmed. Total protein synthesis was determined by the incorporation of [3H]phenylalanine into trichloroacetic acid-precipitable proteins. To study the effect of acute depolarization on protein synthesis, cells were incubated for 30 min with [3H]phenylalanine or [3H]leucine and the depolarizing agent. In fetal cerebrocortical cells, potassium (30 and 56 mM) decreased protein synthesis and RNA levels and increased IR-SRIF release. Depolarization by veratridine, a sodium channel activator, induced a similar effect. The effect of veratridine on IR-SRIF and protein synthesis was reversed by tetrodotoxin, a sodium channel blocker, or verapamil, a calcium channel blocker. These findings suggest that protein synthesis by cerebrocortical cells is decreased in fetal brain cells by membrane depolarization and is dependent on Na+ and Ca2+ entry into cells. In postnatal (day 7) cerebrocortical cells, depolarization induced by high potassium concentrations led to a concomitant increase in protein synthesis, RNA content, and somatostatin release. These findings indicate that depolarization of the cellular membrane is coupled to an increase in protein synthesis in neonatal, but not in fetal, dispersed brain cells.
M T de los Frailes; L Cacicedo; M J Lorenzo; F Sánchez-Franco
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neurochemistry     Volume:  52     ISSN:  0022-3042     ISO Abbreviation:  J. Neurochem.     Publication Date:  1989 May 
Date Detail:
Created Date:  1989-06-02     Completed Date:  1989-06-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1333-9     Citation Subset:  IM    
Servicio de Endocrinologia, Hospital Ramón y Cajal, Madrid, Spain.
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MeSH Terms
Animals, Newborn / physiology*
Calcium / metabolism
Cell Membrane / physiology*
Cells, Cultured
Cerebral Cortex / drug effects,  embryology,  physiology*
Fetus / physiology*
Leucine / metabolism
Membrane Potentials
Nerve Tissue Proteins / biosynthesis*
Phenylalanine / metabolism
Potassium / pharmacology
RNA / biosynthesis
Rats, Inbred Strains
Sodium / metabolism
Somatostatin / secretion*
Tetrodotoxin / pharmacology
Verapamil / pharmacology
Veratridine / pharmacology
Reg. No./Substance:
0/Nerve Tissue Proteins; 4368-28-9/Tetrodotoxin; 51110-01-1/Somatostatin; 52-53-9/Verapamil; 61-90-5/Leucine; 63-91-2/Phenylalanine; 63231-63-0/RNA; 71-62-5/Veratridine; 7440-09-7/Potassium; 7440-23-5/Sodium; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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