Document Detail

Divergence in signaling pathways involved in promotion of cell viability mediated by bFGF, NGF, and EGF in PC12 cells.
MedLine Citation:
PMID:  12834262     Owner:  NLM     Status:  MEDLINE    
We employed a series of inhibitors of intracellular cascade to disclose the precise molecular mechanisms by which basic fibroblast growth factor (bFGF) promotes viability of PC12 cells and compared with nerve growth factor (NGF) and epidermal growth factor (EGF). The MEK 1 and 2 inhibitors, U0126 and PD98059, significantly suppressed cell viability mediated by bFGF in a dose-dependent manner, and to a greater extent compared with EGF and NGF. The degree of MEK dependency for growth factor-mediated cell viability was estimated to be in the order of bFGF, EGF, and NGF. Rapamycin strongly inhibited the effect of NGF on cell viability, compared with bFGF and EGF. The mechanisms of action of NGF-mediated cell viability may depend largely on p70 S6 kinase-related signal transduction pathways comparing to bFGF and EGF. The present findings suggest that different signal transduction systems may be involved in the molecular mechanisms by which bFGF, NGF, and EGF mediate cell viability.
Takakazu Kawamata; Tomoko Yamaguchi; Kazuo Shin-ya; Tomokatsu Hori
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Neurochemical research     Volume:  28     ISSN:  0364-3190     ISO Abbreviation:  Neurochem. Res.     Publication Date:  2003 Aug 
Date Detail:
Created Date:  2003-07-01     Completed Date:  2003-09-03     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  7613461     Medline TA:  Neurochem Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1221-5     Citation Subset:  IM    
Department of Neurosurgery, Neurological Institute, Tokyo Women's Medical University, Tokyo, Japan.
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MeSH Terms
Butadienes / pharmacology
Cell Survival / physiology*
Enzyme Inhibitors / pharmacology
Epidermal Growth Factor / physiology*
Fibroblast Growth Factor 2 / physiology*
Flavonoids / pharmacology
Nerve Growth Factor / physiology*
Nitriles / pharmacology
PC12 Cells
Phosphatidylinositol 3-Kinases / antagonists & inhibitors
Signal Transduction / physiology*
Sirolimus / pharmacology
Reg. No./Substance:
0/2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one; 0/Butadienes; 0/Enzyme Inhibitors; 0/Flavonoids; 0/Nitriles; 0/U 0126; 103107-01-3/Fibroblast Growth Factor 2; 53123-88-9/Sirolimus; 62229-50-9/Epidermal Growth Factor; 9061-61-4/Nerve Growth Factor; EC 2.7.1.-/Phosphatidylinositol 3-Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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