Document Detail

Diuretic activity of squamate mistletoe, Viscum angulatum.
MedLine Citation:
PMID:  20645720     Owner:  NLM     Status:  MEDLINE    
Viscum angulatum Heyne ex DC (Viscaceae) is a leafless hemiparasitic shrub traditionally used in Asian countries for the treatment of hypertension. In the present study, the methanol extract of the whole plant was examined for diuretic activity in rats. The activity was evaluated using parameters such as urine volume after 5, 19, and 24 h and urine sodium, potassium, and chloride concentrations. The extract was further scrutinized for polyphenolic compounds and triterpenoids. The extract demonstrated a significant increase in and dose-dependent effect on urine excretion volume in comparison to the normal group in the tested range of 100-400 mg/kg. The extract demonstrated comparable saluretic and higher natriuretic activity (Na(+)/K(+)) compared to the furosemide treated group. However, the Cl(-)/Na(+) + K(+) ratio, which indicates carbonic anhydrase mediated activity, remained unaffected. HPLC and quantitative analysis of the extract revealed that polyphenolic compounds and the triterpenoid, oleanolic acid, are the major phytochemicals, and are proposed to be responsible for the observed diuretic effect. Oleanolic acid has been reported to possess diuretic activity with significant potassium loss in rats. In contrast to pure oleanolic acid, the extract demonstrated a valuable potassium-sparing effect. This suggests modulation of the diuretic effect of oleanolic acid by polyphenolics present in the extract. The observed dose-dependent potassium-sparing diuretic effect is a hereto unreported property of this plant.
R B Jadhav; S P Bhatnagar; S J Surana
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Pharmaceutical biology     Volume:  48     ISSN:  1744-5116     ISO Abbreviation:  Pharm Biol     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-07-21     Completed Date:  2010-11-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9812552     Medline TA:  Pharm Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  417-21     Citation Subset:  IM    
Bioecology Laboratory, Department of Pharmacognosy, R.C. Patel Institute of Pharmaceutical Education and Research, Shirpur, India.
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MeSH Terms
Acute Toxicity Tests
Chlorides / analysis
Chromatography, High Pressure Liquid
Diuresis / drug effects*
Diuretics / isolation & purification,  pharmacology*,  toxicity
Dose-Response Relationship, Drug
Plant Extracts / isolation & purification,  pharmacology*,  toxicity
Potassium / analysis
Rats, Wistar
Sodium / analysis
Triterpenes / analysis
Urine / chemistry
Viscum / chemistry*
Reg. No./Substance:
0/Chlorides; 0/Diuretics; 0/Plant Extracts; 0/Triterpenes; 7440-09-7/Potassium; 7440-23-5/Sodium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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