Document Detail

Disuse osteopenia is accompanied by downregulation of gene expression for bone proteins in growing rats.
MedLine Citation:
PMID:  1476173     Owner:  NLM     Status:  MEDLINE    
Unilateral sciatic neurectomy (USN) resulted in cortical osteopenia in tibiae from the sciatic nerve-sectioned limb of growing rats. The bone deficit resulted from decreased periosteal addition; there were no changes in the indexes of bone resorption. The periosteal bone formation rate was reduced in the nerve-sectioned limb within 7 days of sciatic neurectomy, and this decrease persisted for at least 56 days. Steady-state mRNA levels for bone proteins were determined in periosteum isolated from tibiae and femurs 7 and 14 days after sciatic nerve section. Nerve section resulted in decreased levels of mRNA for osteocalcin, alkaline phosphatase, and possibly the prepro-alpha (I)-subunit of type I collagen (collagen). The effects were more pronounced in tibiae than femurs, corresponding to the greater degree of immobility induced by USN in the former bone. The results demonstrate that decreased bone formation precedes establishment of disuse cortical osteopenia in growing rats with no evidence for a change in bone resorption. Furthermore, the decreased bone formation is associated with, and may be due to, reduced mRNA levels for matrix proteins and other important bone proteins.
G K Wakley; J S Portwood; R T Turner
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  263     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1992 Dec 
Date Detail:
Created Date:  1993-02-03     Completed Date:  1993-02-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  E1029-34     Citation Subset:  IM; S    
Department of Orthopedic Surgery, Mayo Clinic, Rochester, Minnesota 55905.
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MeSH Terms
Aging / physiology*
Alkaline Phosphatase / genetics
Bone Diseases, Metabolic / etiology*,  genetics,  physiopathology
Bone Resorption
Collagen / genetics*
Denervation / methods
Gene Expression*
Osteocalcin / genetics*
RNA, Messenger / metabolism
Rats, Sprague-Dawley
Sciatic Nerve / physiology
Tibia / growth & development
Grant Support
Reg. No./Substance:
0/RNA, Messenger; 104982-03-8/Osteocalcin; 9007-34-5/Collagen; EC Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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