Document Detail

Distributions of rheological parameters in populations of human erythrocytes.
MedLine Citation:
PMID:  10690266     Owner:  NLM     Status:  MEDLINE    
We have previously proposed the osmofiltration method based on a modified Hanss hemorheometer to analyze distributions of erythrocytes in their ability to pass through membrane filters with 3 microns pores. Upon decrease in medium osmolality (u) the erythrocyte volume increases. When cell volume becomes V = Vcr at u = ucr, such cell loses its ability to pass through a 3 microns pore. The flow rate of erythrocyte suspension containing cells with different ucr through a filter gradually decreases with decreasing medium osmolality. This rate becomes zero at some u = omega, when the number of non-filterable cells in the applied sample approaches the number of pores in filter. Experimental determination of the dependencies of the filtration rate on medium osmolality for various hematocrit values allows to obtain omega for each hematocrit and, thereby, to assess the distribution of erythrocytes in ucr. Here, we propose a simplified version of this method, which allows screening of the erythrocytes in heterogeneous suspensions for the distribution in ucr by measuring omega for only two hematocrit values, 0.1% and 1%. Applications of the proposed method are exemplified by analysing the erythrocyte populations of healthy donors, of patients with microspherocytosis, hemochromatosis and normal erythrocyte populations in an acidic environment.
I L Lisovskaya; E S Shurkhina; E E Yakovenko; N V Tsvetaeva; S V Kolodei; S P Shcherbinina; F I Ataullakhanov
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biorheology     Volume:  36     ISSN:  0006-355X     ISO Abbreviation:  Biorheology     Publication Date:  1999  
Date Detail:
Created Date:  2000-03-09     Completed Date:  2000-03-09     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0372526     Medline TA:  Biorheology     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  299-309     Citation Subset:  IM    
National Research Center for Hematology, Russian Academy of Medical Sciences, Moscow, Russia.
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MeSH Terms
Erythrocyte Deformability*
Erythrocyte Volume
Hemochromatosis / blood
Hydrogen-Ion Concentration
Micropore Filters
Osmolar Concentration
Spherocytosis, Hereditary / blood

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