Document Detail

Distributions of high-density lipoprotein particle components in human follicular fluid and sera and their associations with embryo morphology parameters during IVF.
MedLine Citation:
PMID:  18487218     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: High-density lipoprotein (HDL) is the sole lipoprotein present in follicular fluid (FF). The objectives of this study were to examine HDL lipid composition and associated enzyme activities in FF and serum and to relate these levels to embryo morphology parameters in women undergoing in vitro fertilization (IVF).
METHODS: Serum and FF were prospectively obtained from 60 women undergoing IVF. HDL lipids, apolipoprotein AI (ApoAI), paraoxonase 1 (PON1) and paraoxonase 3 (PON3) activities were determined. Bivariate analysis and ordinal logistic regression models were employed to examine the associations between biochemical measures and embryo morphology parameters [embryo cell number (ECN) and embryo fragmentation score (EFS)] as surrogate markers of oocyte health.
RESULTS: All biochemical parameters were significantly (P < 0.05) lower in FF than serum except PON3 levels which were significantly higher. FF-HDL cholesterol (OR 0.66, 95%CI 0.46-0.96) and ApoAI (OR 0.13, 95%CI 0.03-0.97) levels were negative predictors for EFS; however, their effects were not independent and the level of one moderated the effect of the other. Limited to Day 3 embryo transfers, FF-PON1-arylesterase activity was a significant positive predictor for ECN (OR 1.09, 95%CI 1.01-1.17).
CONCLUSIONS: In this pilot study, our data suggests that HDL and its component proteins within FF may play protective roles in the health of the human oocyte and subsequent early embryo development. We describe for the first time the activities of PON1 and PON3 in FF. We suspect that PON3 activity may be locally generated due to higher activities in FF compared with serum.
R W Browne; W B Shelly; M S Bloom; A J Ocque; J R Sandler; H G Huddleston; V Y Fujimoto
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Publication Detail:
Type:  Journal Article; Twin Study     Date:  2008-05-16
Journal Detail:
Title:  Human reproduction (Oxford, England)     Volume:  23     ISSN:  1460-2350     ISO Abbreviation:  Hum. Reprod.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-07-18     Completed Date:  2008-09-29     Revised Date:  2011-09-09    
Medline Journal Info:
Nlm Unique ID:  8701199     Medline TA:  Hum Reprod     Country:  England    
Other Details:
Languages:  eng     Pagination:  1884-94     Citation Subset:  IM    
Department of Biotechnical and Clinical Laboratory Sciences, University at Buffalo, State University of New York, 26 Cary Hall, 3435 Main Street, Buffalo, NY 14214, USA.
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MeSH Terms
Apolipoprotein A-I / metabolism
Aryldialkylphosphatase / antagonists & inhibitors,  metabolism
Embryo, Mammalian / anatomy & histology*
Esterases / antagonists & inhibitors,  metabolism
Fertilization in Vitro*
Follicular Fluid / chemistry*
Lipoproteins, HDL / blood,  metabolism*
Logistic Models
Prospective Studies
Simvastatin / metabolism
Reg. No./Substance:
0/Apolipoprotein A-I; 0/Lipoproteins, HDL; 79902-63-9/Simvastatin; EC 3.1.-/Esterases; EC; EC protein, human; EC protein, human

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