Document Detail


Distributional and developmental variations of multiple forms of calpastatin in mouse brain.
MedLine Citation:
PMID:  2079637     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
DEAE-cellulose chromatography of mouse brain extract demonstrated the occurrence of two calpastatin fractions, CS-0.1 and CS-0.2, with distinctly higher content of the latter. CS-0.1 emerged from the column at 0.1 M NaCl, inhibited calpain II more strongly than calpain I, and identified also immunologically with hitherto known calpastatin. CS-0.2 emerged at 0.2 M NaCl, inhibited calpain I more strongly than calpain II, and did not crossreact with anti-calpastatin antibody used. Fairly consistent amounts of CS-0.2 and calpain II were found in the brain of mice from 10 days to 10 weeks after birth, while CS-0.1 became measurable only after 4-week growth. In adult mice, CS-0.1 was highest in specific activity in brainstem, lower in cerebellum, and not detectable in cerebral hemisphere. Physiological significance of multiple forms of calpastatin and their variations found is not known.
Authors:
T Hamakubo; M Ueda; E Takano; T Murachi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of enzyme inhibition     Volume:  3     ISSN:  8755-5093     ISO Abbreviation:  J. Enzym. Inhib.     Publication Date:  1990  
Date Detail:
Created Date:  1991-04-29     Completed Date:  1991-04-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8709734     Medline TA:  J Enzyme Inhib     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  203-10     Citation Subset:  IM    
Affiliation:
Department of Clinical Science and Laboratory Medicine, Kyoto University Faculty of Medicine, Japan.
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MeSH Terms
Descriptor/Qualifier:
Aging
Animals
Blotting, Western
Brain / growth & development*
Brain Chemistry
Calcium-Binding Proteins / isolation & purification*,  pharmacology
Calpain / antagonists & inhibitors*
Chromatography, DEAE-Cellulose
Cysteine Proteinase Inhibitors / isolation & purification*
Electrophoresis, Polyacrylamide Gel
Mice
Mice, Inbred A
Molecular Weight
Chemical
Reg. No./Substance:
0/Calcium-Binding Proteins; 0/Cysteine Proteinase Inhibitors; 79079-11-1/calpastatin; EC 3.4.22.-/Calpain

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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