Document Detail

Distributional and developmental variations of multiple forms of calpastatin in mouse brain.
MedLine Citation:
PMID:  2079637     Owner:  NLM     Status:  MEDLINE    
DEAE-cellulose chromatography of mouse brain extract demonstrated the occurrence of two calpastatin fractions, CS-0.1 and CS-0.2, with distinctly higher content of the latter. CS-0.1 emerged from the column at 0.1 M NaCl, inhibited calpain II more strongly than calpain I, and identified also immunologically with hitherto known calpastatin. CS-0.2 emerged at 0.2 M NaCl, inhibited calpain I more strongly than calpain II, and did not crossreact with anti-calpastatin antibody used. Fairly consistent amounts of CS-0.2 and calpain II were found in the brain of mice from 10 days to 10 weeks after birth, while CS-0.1 became measurable only after 4-week growth. In adult mice, CS-0.1 was highest in specific activity in brainstem, lower in cerebellum, and not detectable in cerebral hemisphere. Physiological significance of multiple forms of calpastatin and their variations found is not known.
T Hamakubo; M Ueda; E Takano; T Murachi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of enzyme inhibition     Volume:  3     ISSN:  8755-5093     ISO Abbreviation:  J. Enzym. Inhib.     Publication Date:  1990  
Date Detail:
Created Date:  1991-04-29     Completed Date:  1991-04-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8709734     Medline TA:  J Enzyme Inhib     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  203-10     Citation Subset:  IM    
Department of Clinical Science and Laboratory Medicine, Kyoto University Faculty of Medicine, Japan.
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MeSH Terms
Blotting, Western
Brain / growth & development*
Brain Chemistry
Calcium-Binding Proteins / isolation & purification*,  pharmacology
Calpain / antagonists & inhibitors*
Chromatography, DEAE-Cellulose
Cysteine Proteinase Inhibitors / isolation & purification*
Electrophoresis, Polyacrylamide Gel
Mice, Inbred A
Molecular Weight
Reg. No./Substance:
0/Calcium-Binding Proteins; 0/Cysteine Proteinase Inhibitors; 79079-11-1/calpastatin; EC 3.4.22.-/Calpain

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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