Document Detail


Distribution of uidA gene sequences in Escherichia coli isolates in water sources and comparison with the expression of beta-glucuronidase activity in 4-methylumbelliferyl-beta-D-glucuronide media.
MedLine Citation:
PMID:  8357258     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The uidA gene, which encodes the beta-glucuronidase enzyme, was detected in 97.7% of 435 Escherichia coli isolates from treated and raw water sources by DNA-DNA hybridization; 92.4% of the strains expressed the translational product in 4-methylumbelliferyl-beta-D-glucuronide-containing media after reinoculation. Upon initial isolation from water samples, the minimal medium o-nitrophenyl-beta-D-galactopyranoside-4-methylum-belliferyl -beta-D-glucuronide preparations failed to detect more than 50% of the E. coli isolates that possessed uidA gene. Treated water gave the lowest recovery, with Colilert producing 26% positive samples and Coliquik producing 48% positive samples. There appears to be no relationship between the intensity of the autoradiographic signals of the uidA gene and the expression of beta-glucuronidase activity. Therefore, another variable such as physiological condition of the bacteria could be responsible for the nonexpression of the enzyme activity.
Authors:
M T Martins; I G Rivera; D L Clark; M H Stewart; R L Wolfe; B H Olson
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  59     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1993 Jul 
Date Detail:
Created Date:  1993-09-17     Completed Date:  1993-09-17     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2271-6     Citation Subset:  IM    
Affiliation:
Department of Microbiology, University of Sao Paulo, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Autoradiography
Culture Media / chemistry
Escherichia coli / enzymology,  genetics*
False Negative Reactions
Glucuronidase / genetics,  metabolism*
Hymecromone / analogs & derivatives*
Water Microbiology*
Chemical
Reg. No./Substance:
0/Culture Media; 6160-80-1/4-methylumbelliferyl glucuronide; 90-33-5/Hymecromone; EC 3.2.1.31/Glucuronidase
Comments/Corrections

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